Using the yeast resting cells and macroporous adsorptive resins respectively as enzyme carriers and separation medium, continuous transformation of 2-phenylethanol was carried out with the aid of in situ product removal techniques. Meanwhile, the strains were separated through microfiltration membrane. The transformation conditions were optimized in 5 L fermentor, and continuous bioconversion of 2-phenylethanol was fulfilled by feeding and controlling product concentration. The concentration of 2-phenylethanol was 24.06 g/L after running 144 h in single tank. The molar conversion rate was 79.34 %, and the average space-time yield was 0.166 g/(L·h). After running 144 h in double tanks, the concentration of 2-phenylethanol reached 29.86 g/L, which was higher than single tank production by 24.1%. The molar conversion rate and the average space-time yield were up to 81.83 % and 0.204 6 g/(L·h), respectively. After repeated use of resting cells for 10 times, the production and average conversion rate of 2-PE did not decrease obviously.