The strain of high yield glycosylated enzyme was screened from the wine, and the tolerance study of the screened strains and the optimization of the enzymatic conditions of the solid fermentation were carried out. Tolerance test results showed that the strain was tolerant of 10% alcohol and the pH 4 above, and its growth was significantly inhibited at pH 3.5, while it could grow better at 23-41 ℃. By single factor test and orthogonal test, the best conditions for solid-state fermentation determined as follows: enzyme addition of 4%, bran shell quantity of 1.25%, initial pH value of 5.5, water ratio 5∶3 (g∶mL), cultivation at 30 ℃ for 72 h. Under these conditions, the saccharifying enzyme activity was up to 1791.3 U/g. The strain was made into a light disc. The appearance was in accordance with the requirements, and the content of sugar was 24.76 g/100g.