Phenyllactic acid is an important organic acid that has been widely used in foods, pharmaceuticals, cosmetics and other fields. D-phenyllactic acid was catalytically synthesized by whole cells through constructing recombinant Escherichia coli BL21 (DE3)/pET28a-ldh D. The induction and transformation conditions were optimized and determined as follows: IPTG was added to a final concentration of 0.2 mmol/L when OD600 reached 0.8 and cells were induced at 25 ℃ for 4 h. The optimal transformation conditions were as follows: using pH=7.0 phosphate buffer, 13 g/L sodium phenylpyruvate, 5 g/L glucose, 30 g/L cells (dry weight), at 37 ℃ and 200 r/min for 2 h. Under the optimal conditions, phenyllactic acid accumulated up to 5.2 g/L and the yield was 40%. Effects of fed-batch process using sodium pyruvate and glucoase on phenyllactic acid synthesis were explored, and the best processing scheme was obtained. After 3 h of conversion, the concentration of D-phenyllactic and its yield increased to 7.38 g/L and 52.7%, respectively. This study showed that the process of whole cell transformation for D-phenyllactic acid synthesis had good application prospects, which laid a foundation for subsequent enzymatic modification.
BAO Zhiwei
,
SU Xiao
,
YANG Liuting
,
CHEN Yao
,
LUO Xi
,
FU Yongqian
,
SUN Xiaolong
. Biocatalytic production of D-phenyllactic acid by using whole cells of recombinant Escherichia coli [J]. Food and Fermentation Industries, 2019
, 45(1)
: 49
-53
.
DOI: 10.13995/j.cnki.11-1802/ts.017960
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