Food and Fermentation Industries >

2019 , Vol. 45 >Issue 1: 236 - 245

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.018496

Research progress on detecting aflatoxins in foods using fluorescence immunoassay

  • ZUO Xiaowei ,
  • LEI Lin ,
  • LIU Hebing ,
  • TAO Xiaoqi
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  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Beijing WDWK Biotech Co., Ltd, Beijing 100095, China)
    3(Chongqing Engineering Research Center of Regional Food, Chongqing 400715, China)

Received date: 2018-08-11

  Online published: 2019-02-01

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Abstract

Aflatoxins are the most toxic toxins among all well-known mycotoxins. They are recognized as a Class I carcinogen, and are widely present in grains, nuts, oils, milk and their products. Therefore, developing an accurate, rapid, and efficient detection method is of great significance for ensuring food safety and for human health. Fluorescence immunoassay has advantages of high sensitivity, good accuracy, fast detection, and simple operation. Therefore, it is very suitable for on-site high-throughput rapid screening. In recent years, it has been increasingly used for detecting aflatoxins in foods. In this review, four commonly used tracers (fluorescein, quantum dots, upconverting nanoparticles, and rare earth ion chelates) were introduced. Then, principles of five fluorescence immunoassays (fluorescence-linked immunosorbent assay, fluorescence immunochromatographic assay, fluorescence polarization immunoassay, fluorescence resonance energy transfer immunoassay, and time-resolved fluoroimmunoassay) were reviewed. Besides, the latest research progress on detecting aflatoxins in foods using these methods nationally and internationally were emphasized to provide guidance for future researches.

Key words： aflatoxin; foods; fluorescent tracer; fluorescence immunoassay

Cite this article

ZUO Xiaowei , LEI Lin , LIU Hebing , TAO Xiaoqi . Research progress on detecting aflatoxins in foods using fluorescence immunoassay[J]. Food and Fermentation Industries, 2019 , 45(1) : 236 -245 . DOI: 10.13995/j.cnki.11-1802/ts.018496

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