The α-amylase gene (McAmyA) from Malbranchea cinnamomea was successfully cloned and heterologously expressed in Pichia pastoris GS115. The recombinant α-amylase (McAmyA) was secreted at high level of 13440.6 U/mL by high cell density fermentation after 168 h. McAmyA was purified by QSFF ion-exchange chromatography with specific activity of 1230.2 U/mg. The optimal pH and temperature of McAmyA were determined to be 6.5 and 65 ℃, respectively. The maltose syrup with maltose content of 50% was prepared at 60 ℃, 120 U/g and 24 h. The fungus α-amylase was highly expressed in Pichia pastoris and it has great potential for application.
ZHAO Ning
,
WANG Yuchuan
,
YI Ping
,
YAN Qiaojuan
,
JIANG Zhengqiang
. High level expression of α-amylase from Malbranchea cinnamomea in Pichia pastoris and preparation of maltose syrup[J]. Food and Fermentation Industries, 2019
, 45(2)
: 1
-6
.
DOI: 10.13995/j.cnki.11-1802/ts.016703
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