To purify peptides with strong antioxidant activities, carrot seed protein hydrolysate was fractionated using size exclusion chromatography and it was further purified by RP-HPLC. Antioxidant activities of peptides were evaluated by DPPH radical scavenging activity. Two fractions, F3-a and F3-b, with higher DPPH scavenging activity were purified. Their sequences were identified as Lys-Asp-Asn-Phe-Leu-Phe (KDNFLF) and Leu-Phe (LF), respectively, by LC-ESI-MS/MS analysis. Moreover, the scavenging activities for ABTS radical and superoxide anion radical, as well as linoleic acid peroxidation inhibition activity were studied. Both KDNFLF and LF showed strong radical scavenging activities. These results suggested that the purified carrot seed antioxidant peptides possess good antioxidant activities and they could be used as potential antioxidants in the food and pharmaceutical industries.
WANG Huimin
,
HU Pei
,
CAI Tiantian
,
XU Shuli
,
HONG Jing
,
WANG Shaoyun
. Isolation, identification and characterization of antioxidative carrot seed peptides[J]. Food and Fermentation Industries, 2019
, 45(2)
: 95
-100
.
DOI: 10.13995/j.cnki.11-1802/ts.017302
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