In order to distinguish walnuts according to their geographic origin, 344 samples of walnuts from Henan, Xinjiang, Hebei, Gansu, Shanxi, Sichuan, Hubei, Yunnan and Guangxi were collected. Their isotope ratios of C, H, O, N were determined by isotope ratio mass spectrometry. The N content was measured by elemental analyzer and it was utilized to calculate the corresponding crude protein content. The crude fat content was determined by physicochemical analytical method. The results showed that δ2H and δ18O were reduced with decreased latitude. The δ13C value of walnuts in Henan sub-region increased with elevated latitude. The linear discriminant analysis found that the overall rates of correctly discriminating walnuts from different provinces and Henan sub-region by the stable isotope ratio of C、H、O、N were 71.1% and 57.9%, respectively. When combined with the contents of crude fat and protein, the overall rates of correct discrimination increased up to 79.6% and 68.6%, respectively. It was found that stable isotopes in combination with organic component analysis could help discriminating the origins of walnuts. In addition, the discrimination rates for discriminating walnuts from different provinces are higher than those for sub-regions.
WANG Pengfei
,
GUO Kang
,
SHEN Juanzhang
,
TAN Weihong
. Characterise the geographical origin of walnut using stable isotopes in combination with crude fat and protein content[J]. Food and Fermentation Industries, 2019
, 45(2)
: 214
-219
.
DOI: 10.13995/j.cnki.11-1802/ts.017414
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