The scavenging effects of twenty-four strains of Lactobacillus on hydroxyl radicals, DPPH free radicals, and their reducing activities were investigated to compare their antioxidative activities in vitro. Then, Caenorhabditis elegans fed with these twenty-four Lactobacillus strains were put into a 0.003%(mass froution) hydrogen peroxide solution that induced oxidative stress. By comparing the lifespan of C. elegans fed with different Lactobacillus strains, abilities of Lactobacilli to mitigate oxidative damages in C. elegans were analyzed. The results showed that L. plantarum Lp11 not only exhibited high antioxidant capacity in vitro, but also effectively alleviated the oxidative stress in C. elegans with the best effect. The correlation analysis between antioxidant indexes in vitro and LT50 of C. elegans showed that hydroxyl radical scavenging capacity had a strong correlation with oxidative stress alleviation in C. elegans (P<0.01). Therefore, hydroxyl radical scavenging capacity of Lactobacilli can be used to reflect the antioxidant activity of Lactobacilli in C. elegans.
NI Caixin
,
JIN Xing
,
ZHOU Wei
,
CHEN Xiaohua
,
YIN Boxing
,
FANG Dongsheng
,
WANG Gang
,
ZHAO Jianxin
,
ZHANG Hao
,
CHEN Wei
. Evaluation of antioxidant activity of Lactobacillus in vivo using Caenorhabditiselegans and comparison with its antioxidant parameters in vitro[J]. Food and Fermentation Industries, 2019
, 45(3)
: 21
-27
.
DOI: 10.13995/j.cnki.11-1802/ts.018228
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