Food and Fermentation Industries >

2019 , Vol. 45 >Issue 3: 47 - 55

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.018491

High-throughput sequencing analysis of microbial diversity in the combinedfermentation stages of cow dung and corn straw

  • WANG Xuhui ,
  • XU Xin ,
  • BAO Zhe ,
  • WANG Hui ,
  • YE Kai ,
  • LI Guan ,
  • DENG Yu
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  • 1 (College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China)
    2 (Organisms Energy Research Institute, Xinjiang Academy of Agriculture Science, Urumqi 830091, China)
    3 (Institute of Food Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China)
    4 (Rural Energy and Environment Agency, Ministry of Agriculture and Rural Affairs, Beijing 100125, China)
    5 (Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China)

Received date: 2017-12-26

  Online published: 2019-03-11

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Abstract

Effects of different proportions of microbial agents on the fermentation of cow dung-corn straw combined with biogas fermentation were compared, the high-throughput sequencing technology was used to analyze the microbial diversity in the biogas fermentation broth with the best bacteria. The results showed that the optimal condition to ferment corn straw and cow dung was as follows: microbial agents were Bacillus cereus, Pseudomonas formosensis, Bacillus subtilis, Pseudomonas songnenensis (mass ratio 3∶2∶3∶2). Under these conditions, the highest biogas and methane yield reached 72 mL/g VS and 39 mL/g VS while the highest cumulative biogas and methane yield reached 778 mL/g VS and 532 mL/g VS, respectively. The high-throughput sequencing results showed that Bacteroidetes were the most abundant bacteria and was the most important group of the dominant bacterial species. The relative abundance trend in the biogas fermentation process increased from 33.8% during the early stage of fermentation to 43.6% at the end stage of fermentation. Ascomycota was the most abundant among the fungi. The main dominant group in the biogas fermentation process first increased and then decreased, went from 65.7% during the initial fermentation stages to 84.7% in the middle of the fermentation process and dropped to 66.8% at the end of the fermentation process. The research on combined fermentation of cow dung and corn straw provides a theoretical basis for preparing a biogas agent.

Key words： biogas; corn straw; fungal diversity; high-throughput sequencing

Cite this article

WANG Xuhui , XU Xin , BAO Zhe , WANG Hui , YE Kai , LI Guan , DENG Yu . High-throughput sequencing analysis of microbial diversity in the combinedfermentation stages of cow dung and corn straw[J]. Food and Fermentation Industries, 2019 , 45(3) : 47 -55 . DOI: 10.13995/j.cnki.11-1802/ts.018491

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