The crude carotenoid extracts from Acinetobacter lwoffii UL fermentation were purified by thin layer chromatography (TLC) and silica gel column chromatography to obtain component 1 and component 2. Solubility test, high performance liquid chromatography (HPLC), fourier transform infrared spectrometer (FTIR), 1 hydrogen-nuclear magnetic resonance (1H-NMR), and UPLC-Q-TOF-MS-MS were used to identify component 2. The results showed that pigments in component 2 were fat soluble and had carotenoid characteristic structure, such as methyl , methylene, polyene long chain, benzene ring, and carbonyl group. It was preliminarily deduced that component 2 contained astacen (C40H48O4, molecular weight 592) and few astaxanthin (C40H52O4, molecular weight 596). ABTS, FRAP, and DPPH were used to evaluate the antioxidant activities of carotenoid crude extracts, purified component 1 and component 2. The results showed that carotenoid crude extracts had antioxidant activities, and the antioxidant activities of purified carotenoids increased significantly.
SHENG Ran
,
SUN Zhigao
,
ZHANG Zhen
,
FANG Ming
,
YU Fengsheng
,
ZHANG Qunlin
,
LI Tian
. Purification and identification of carotenoids from Acinetobacter lwoffii UL andtheir antioxidant activities[J]. Food and Fermentation Industries, 2019
, 45(3)
: 95
-102
.
DOI: 10.13995/j.cnki.11-1802/ts.018282
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