The effects of four different surfactants,Tween-80, Triton X-100, CTAB, and EMB, on cytidine synthesis by fermentation with Escherichia coli BG-12 as well as cell grouth were studied. The yield of cytidine increased while 1-4 g/L Tween-80 was added. The maximum yield of 1.7 g/L was obtained when 3 g/L of Tween-80 was added, which was 2.42 times higher than that of the control. Additionally, it had no significant effects on bacteria growth and sugar consumption. When TritonX-100 was added, the yield of cytidine decreased, however, 0.4-0.8 g/L TritonX-100 could promote bacteria growth and sugar consumption. When CTAB or EMB was added, the yield of cytidine decreased obviously, but had little effects on bacteria growth and sugar consumption. In conclusion, the yield of cytidine increased when less than 4 g/L Tween-80 was added after 10 h fermentation. In comparison, TritonX-100, CTAB, and EMB inhibited cytidine production, although they might stimulate cell growth.
ZHAO Beibei
,
FANG Haitian
,
LIU Huiyan
,
MA Ruoshuang
,
WANG Ru
,
LIU Yuxuan
,
LI Jinna
. Effects of different surfactants on cytidine production by fermentation[J]. Food and Fermentation Industries, 2019
, 45(4)
: 48
-54
.
DOI: 10.13995/j.cnki.11-1802/ts.018457
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