This study was conducted to optimize the saponification process of vitamin D2(VD2)in white button mushrooms by response surface methodology after irradiating the mushrooms by UV-C. The VD2 content was measured by high performance liquid chromatography. Volume ratio of alcohol to alkali was determined by single factor experiments. The liquid to solid ratio, saponification temperature, and saponification time were taken as independent variables, and the amount of VD2 extracted was the response value. The effects of various factors on extracting VD2 and the optimum process parameters were determined by the response surface methodology. The results showed that the best saponification process of VD2 in white button mushrooms was as follows: the volume ratio of alcohol to alkali was 2∶1(V∶V), the liquid-solid ratio was 12∶1(mL∶g), the saponification temperature was 75 ℃, and the saponification time was 63 min. Under this condition, the amount of VD2 extracted was 8.13 μg/g, which was similar to the theoretical value. The established saponification process is practical, and it provides a theoretical reference for the development and utilization of VD2 in the mushroom.
ZHANG Yue
,
SHEN Yaping
,
LI lili
,
WANG Fengling
,
GUAN Wenqiang
. Optimization of the saponification process of vitamin D2 in white button mushrooms after UV-C irradiation by response surface methodology[J]. Food and Fermentation Industries, 2019
, 45(4)
: 181
-186
.
DOI: 10.13995/j.cnki.11-1802/ts.018127
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