Effect of Bacillus pumilus HN-10 antimicrobial peptide P-2 on preserving Agaricus bisporus

  • NIU Yaoxing ,
  • YUN Jianmin ,
  • LI Yanhu ,
  • GUO Juan ,
  • DENG Zhanrui ,
  • LIU Xiaoxia
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  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070,China)

Online published: 2019-03-25

Abstract

In order to prevent mushroom spoilage caused by postharvest microbial infection and prolong its shelf-life, effects of Bacillus pumilus HN-10 antimicrobial peptide P-2 on preserving Agaricus bisporus were studied. Fresh Agaricus bisporus was used as raw material, antimicrobial peptide P-2 extracted from fermentation broth of B. pumilus HN-10 was used as the preservative and sprayed to Agaricus bisporus with different concentrations(the gradient dilution ratio was 1∶1 ,1∶2, and 1∶3(V/V), using sterile water). The optimal concentration of antimicrobial peptide P-2 was selected at room temperature (25±1)℃, and the preservation and storage experiments were conducted at low temperature (4±1)℃. The results showed that the optimal dilution ratio was 1∶1 (V/V) at room temperature. After storing for 3 days, treated mushrooms still maintained certain sensory qualities and their decay indexes were relatively low. The antimicrobial peptide P-2 could significantly inhibit the increase of total number of bacterial colonies of Agaricus bisporus, reduce the weight loss, maintain a stable soluble protein content, and effectively inhibit PPO activity under low-temperature storage conditions. After 5 days of storage, treated mushrooms still maintained good sensory qualities and certain commercial values. Therefore, low temperature storage (4±1)℃ together with antimicrobial peptides P-2 treatment had good preservative effects on Agaricus bisporus and prolonged its shelf-life, which could provide technical reference for developing antimicrobial peptides preservatives for edible mushrooms.

Cite this article

NIU Yaoxing , YUN Jianmin , LI Yanhu , GUO Juan , DENG Zhanrui , LIU Xiaoxia . Effect of Bacillus pumilus HN-10 antimicrobial peptide P-2 on preserving Agaricus bisporus[J]. Food and Fermentation Industries, 2019 , 45(5) : 133 -139 . DOI: 10.13995/j.cnki.11-1802/ts.018917

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