When brewing Gujing Tribute liquor, a large number of Lactobacillus acetotolerans were found in both pitmud and Zaopei. Therefore, it is important to rapidly detect L. acetotolerans specifically. In this paper, whole genome sequences of Lactobacillus bacteria and PRIMER-BLAST method were used to design L. acetotolerans-specific primers. Two pairs of primers were confirmed with decent specificity and combined with quantitative polymerase chain reaction (qPCR) to rapidly measure the amount of L. acetotolerans with different qualities and at different fermentation stages in pitmud and Zaopei. The results showed that the population of L. acetotolerans in Gujing Tribute pitmud was apparently lower than that of Zaopei and was apparently lower in old pitmud than that in pitmud of new plant. Moreover, L. acetotolerans in Zaopei from both new and old plants were generally high with apparent fluctuations among samples. In general, QPCR mediated by L. acetotolerans-specific primers can be used for L. acetotolerans quantification and facilitate quality assessment of pitmud or Zaopei in strong-aroma liquor production.
LIU Qianqian
,
LI Junwei
,
CAO Runjie
,
ZHANG Huimin
,
HE Hongkui
,
LI Anjun
,
ZHANG Zhizhou
. Employment of PRIMER-BLAST to design species-specific primers for Lactobacillus acetotolerans and their implications in quality assessment of pitmud or Zaopei samples in Gujing Tribute liquor[J]. Food and Fermentation Industries, 2019
, 45(6)
: 16
-22
.
DOI: 10.13995/j.cnki.11-1802/ts.019007
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