The gene encoding actin was cloned from Lycium barbarum Bianguo and checked to be a potential reference gene to analyze the expression levels of other genes. The primers were designed based on conserved sequences of actin genes from other Solanaceae plants. The total RNA isolated from leaves of Lycium barbarum Bianguo was used as a template for reverse transcription-polymerase chain reaction (RT-PCR). The nucleotide fragment was amplified and cloned into pMD18-T. The positive clones were identified by PCR, followed by sequencing. The results revealed that the amplified fragment, named as LbACT, contained 598 bp, encoded 198 amino acid residues. The nucleotide sequence was 97% similar to that of Lycium chinense actin gene. Meanwhile, fluorescent quantitative PCR showed that salt-treated LbACT was stable in all organs. Therefore, LbACT could be used as a reference gene to analyze the expressions of functional genes of Lycium barbarum Bianguo.
YUAN Huijun
,
LI Xueyong
,
GAO Ze
,
WANG Chunmei
,
LI Hujun
. Molecular cloning and expression analysis of an actin from Lycium barbarum Bianguo[J]. Food and Fermentation Industries, 2019
, 45(6)
: 48
-53
.
DOI: 10.13995/j.cnki.11-1802/ts.018995
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