In order to reveal the microbial flora structure of oil-sand bean paste stuffing snowy moon cake, the flora were analyzed by high-throughput sequencing and traditional culture isolation. High-throughput sequencing fully reflected the members and abundance of the flora, which was dominated by the species from genera of Cyanobacteria, Bacillus and Staphylococcus. With the extension of storage time, the total number of colonies, Bacillus, and molds increased significantly (P<0.05), while Staphylococcus showed a significant decrease trend. There were fifty-five strains of bacteria and six strains of molds isolated by traditional culture methods, with Bacillus (58.18%) and Staphylococcus (12.72%) had higher ratio, as well as 33.33% Mucor, 16.67% Penicillium, and 50% Aspergillus. It was found that spoilage bacteria were mainly from raw material powders and fillings. This research can provide reference for the safety control of snowy moon cake.
RUAN Zheng
,
WEI Li
,
ZHANG Yanjie
,
XIA Yu
,
LI Biansheng
. Analysis of microbial flora diversity of oil bean paste stuffing snow moon cake at room temperature by high-throughput sequencing combined with traditional culture isolation[J]. Food and Fermentation Industries, 2019
, 45(6)
: 203
-208
.
DOI: 10.13995/j.cnki.11-1802/ts.019386
[1] 张宏康,黄育杭,李笑颜,等.冰皮月饼加工研究进展[J].食品工业,2018,39(7):275-278.
[2] 曾习.速冻冰皮月饼皮的工艺研究[J].食品科技,2017,42(4):157-161.
[3] HUISINTVELD J H.Microbial and biochemical spoilage of foods:an overview[J].International Journal of Food Microbiology,1996,33(1):1-18.
[4] ENGLER K,PALSSON B O.A road map for the development of community systems(Co Sy)biology[J].Nature Review Microbiology,2012,10(5):366-372.
[5] LEDERBERG J.Infectious history[J].Science,2000,288(5 464):287-293.
[6] NERSS, GOODIN D B, PIELAK G J, et al.A rapid droplet method for Sanger dideoxy sequencing[J].Biotechniques,1988,6(5):408-412.
[7] BROEKAERT K, HEYNDRICKX M, HERMAN L, et al. Seafood quality analysis: Molecular identification of dominant microbiota after ice storage on several general growth media[J].Food Microbiology, 2011,28(6): 1 162-1 169.
[8] JIANG Yun,GAO Feng,XU Xinglian, et al. Changes in the composition of the bacterial flora on tray-packaged pork during chilled storage analyzed by PCR-DGGE and real-time PCR[J]. Journal of Food Science, 2011, 76(1):27-33.
[9] 曹荣,张井,孟辉辉,等.高通量测序与传统纯培养方法在牡蛎微生物群落分析中的应用对比[J].食品科学,2016,37(24):137-141.
[10] 曹荣,刘淇,赵玲,等.基于高通量测序的牡蛎冷藏过程中微生物群落分析[J].农业工程学报,2016,32(20):275-280.
[11] 黄恺婷.广式月饼腐败霉菌分离与控制研究[D].广州:华南理工大学,2012.
[12] 陈盼. 广式月饼中微生物区系的研究[D].重庆:西南大学,2009.
[13] 顾媛,贺稚非,李洪军,等.肉制品月饼的细菌及霉菌的检测研究[J].食品科学,2008,29(11):555-558.
[14] WAITE J G, JONES J M, YOUSEF A E.Isolation and identification of spoilage microorganisms using food-based media combined with rDNA sequencing: ranch dressing as a model food[J]. Food Microbiology, 2009, 26 (3):235-239.
[15] MATAMOROS S, PILET M F, GIGOUT F,et al.Selection and evaluation of seafood-borne psychrotrophic lactic acid bacteria as inhibitors of pathogenic and spoilage bacteria[J]. Food Microbiology, 2009, 26 (6):638-644.
[16] 黄林,陈全胜,张燕华,等.冷却猪肉优势腐败菌分离鉴定及致腐能力测定[J].食品科学,2013,34(1):205-209.
[17] 唐文静,王楚文,柳云龙,等.冷藏海鲈鱼优势腐败菌的筛选和鉴定[J].食品科学,2016,37(3):170-174.
[18] JUSTYNA P, REBECCHI A, PISACANE V, et al.Bacterial diversity in typical Italian salami at different ripening stages as revealed by high-throughput sequencing of 16S rRNA amplicons[J]. Food Microbiology,2015,46:342-356.
[19] BERRY D,MAHFOUDH B K,WAGNER M,et al.Barcoded primers used in multiplex amplicon pyrosequencing bias amplification[J]. Applied and Environmental Microbiology,2012,78(2):612.
[20] BARTRAM A K, LYNCH M D, STEARNS J C, et al.Generation of multimillion-sequence 16S rRNA gene libraries from complex microbial communities by assembling paired-end illumina reads[J].Applied & Environmental Microbiology, 2011, 77 (11):3 846-3 852.
[21] CRISAFI F, DENARO R, GENOVESE M, et al. Comparison of 16SrDNA and toxR genes as targets for detection of Vibrio anguillarum in Dicentrarchus labrax kidney and liver[J]. Research in Microbiology,2010,162(3): 223-230.
[22] TOURNIER E, AMENC L, PABLO A L, et al. Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents[J]. Methodsx,2015,2(2):182-191.
[23] FU Zeyu,SONG Jiancheng,JAMESON P E.A rapid and cost effective protocol for plant genomic DNA isolation using regenerated silica columns in combination with CTAB extraction[J]. Journal of Integrative Agriculture,2017,16(8):1 682-1 688.
[24] 吴银宝,史金才,莫测辉,等.猪粪和土壤样品中微生物DNA提取方法的比较[J].农业工程学报,2006(S2):10-13.
[25] 李晓然,龚福明,李洁,等.基于混合培养和高通量测序分析云南传统发酵豆豉中活性细菌群落[J].食品科学,2014,35(7):90-94.