In order to reveal the microbial flora structure of oil-sand bean paste stuffing snowy moon cake, the flora were analyzed by high-throughput sequencing and traditional culture isolation. High-throughput sequencing fully reflected the members and abundance of the flora, which was dominated by the species from genera of Cyanobacteria, Bacillus and Staphylococcus. With the extension of storage time, the total number of colonies, Bacillus, and molds increased significantly (P<0.05), while Staphylococcus showed a significant decrease trend. There were fifty-five strains of bacteria and six strains of molds isolated by traditional culture methods, with Bacillus (58.18%) and Staphylococcus (12.72%) had higher ratio, as well as 33.33% Mucor, 16.67% Penicillium, and 50% Aspergillus. It was found that spoilage bacteria were mainly from raw material powders and fillings. This research can provide reference for the safety control of snowy moon cake.
RUAN Zheng
,
WEI Li
,
ZHANG Yanjie
,
XIA Yu
,
LI Biansheng
. Analysis of microbial flora diversity of oil bean paste stuffing snow moon cake at room temperature by high-throughput sequencing combined with traditional culture isolation[J]. Food and Fermentation Industries, 2019
, 45(6)
: 203
-208
.
DOI: 10.13995/j.cnki.11-1802/ts.019386
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