There were 83 strains screened out from the medium using curcumin (CUR) as the sole carbon source, and they were evaluated by high performance liquid chromatography (HPLC). There were five strains that were able to produce dihydrocurcumin (DHC) and tetrahydrocurcumin (THC) using curcumin as substrate. The strain with the highest initial yield was used as target and was identified as Cyberlindnera rhodanensis by morphological characteristics and 26S rDNA D1/D2 region gene sequence alignment. The results showed that the optimal amounts of carbon source, nitrogen source, and inorganic salts to produce DHC were 40 g/L glucose, 30 g/L peptone, and 2 g/L K2HPO4, respectively. Under this condition, the yield of DHC increased from 10.49% to 20.20%. The optimal amounts of carbon source, nitrogen source, and inorganic salts to produce THC were 20 g/L glucose, 20 g/L peptone, and 3 g/L K2HPO4, respectively. The optimized yield of THC increased by 24.31% from 13.67%. This study screened a yeast strain that can use CUR as a substrate to obtain DHC and THC by microbial transformation for the first time, together with providing new strain resources.
WU Chuanchao
,
XU Fucheng
,
GU Qiuya
,
YU Xiaobin
. Screening and identification of strains transforming curcumin hydrogenated derivatives and medium optimization[J]. Food and Fermentation Industries, 2019
, 45(7)
: 45
-51
.
DOI: 10.13995/j.cnki.11-1802/ts.019321
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