A method that aimed to identify anthocyanins in red wines was established by high-performance liquid chromatography-electrospray ionization tandem triple quaternary mass spectrometry (UPLC-ESI-QQQ) and multi-reaction monitoring (MRM). Five basic anthocyanins in grapes and wines were used as standards. The results found that the five basic anthocyanins were clearly separated within 15 minutes by Poroshell 120 EC-C18 column. The mobile phase A was formic acid: water (0.2:100, volume fraction) and mobile phase B was formic acid: methanol: acetonitrile (0.2:50:50, volume fraction). Under this condition, a positive linear relationship was established between concentration and peak area (R2>0.9983). The recovery rate was 83.38%-118.71%. The RSDs of intra-day and inter-day precisions were 0.62%-1.70% and 2.13%-3.90%, respectively. Meanwhile, there were 45 anthocyanins accurately identified from different wine samples within 28 min, which included 25 monoglycosides and 20 pyranoid glucosides. This method is rapid, simple, and accurate, and can be used for qualitative and quantitative analysis of anthocyanins in red wines.
CHEN Xinran
,
ZHANG Bo
,
ZHANG Huan
,
XIE Yingshuang
,
Wang Bo
,
ZHOU Xiaoping
,
LI Min
,
HAN Shunyu
. Determination of anthocyanins in red wine by ultra-high performance liquid chromatography tandem triple quaternary mass spectrometry[J]. Food and Fermentation Industries, 2019
, 45(7)
: 262
-268
.
DOI: 10.13995/j.cnki.11-1802/ts.018817
[1] 刘丽媛,苑伟,刘延琳. 红葡萄酒中花色苷辅助成色作用的研究进展[J]. 中国农业科学, 2010, 43(12): 2 518-2 526.
[2] GORDILLO B, RODR GUEZ-PULIDO F J, ESCUDERO-GILETE M L, et al. Comprehensive colorimetric study of anthocyanic copigmentation in model solutions. Effects of pH and molar ratio [J]. Journal of Agricultural and Food Chemistry, 2012, 60(11): 2 896-2 905.
[3] 韩富亮, 李杨,李记明,等. 红葡萄酒花色苷结构和颜色的关系研究进展[J]. 食品与生物技术学报, 2011, 30(3): 328-336.
[4] IBERN-GÓMEZ M, ANDRES-LACUEVA C, LAMUELA-RAVENTOS R M, et al. Rapid HPLC analysis of phenolic compounds in red wines[J]. American Journal of Enology and Viticulture, 2002, 53(3): 218-221.
[5] ALCALDE-EON C, ESCRIBANO-BAILON M T, SANTOS-EUELGA C, et al. Changes in the detailed pigment composition of red wine during maturity and ageing: A comprehensive study[J]. Analytica Chimica Acta, 2006, 563(1/2): 238-254.
[6] 冯靖, 时玉正,邱晓,等. 葡萄皮天然色素的提取工艺及其稳定性研究[J]. 食品研究与开发, 2018, 39(4): 60-68.
[7] 刘冰, 葛谦,张艳,等. HPLC法同时测定葡萄和葡萄酒中6种基本花色苷[J]. 中国酿造, 2017, 36(2): 162-165.
[8] 李琪, 李广,金丽琼,等. HPLC法测定甘肃地产不同品种酿酒葡萄中的花色苷[J]. 中国酿造, 2014,33(3): 132-136.
[9] LI S Y, HE F, ZHU B Q, et al. A systematic analysis strategy for accurate detection of anthocyanin pigments in red wines[J]. Rapid Communications in Mass Spectrometry 2016, 30(13): 1 619-1 626.
[10] EUGENIO R A.Value of high-performance liquid chromatographic analysis of anthocyanins in the differentiation of red grape cultivars and red wines made from them[J]. Journal of Chromatography A, 2001, 88(915): 53-60.
[11] 邓欣, 蒋娅兰,黄芳,等. 亚3微米色谱分离-串联质谱法同时筛查中药材中非法添加的30种人工色素[J]. 分析试验室, 2018, 37(4): 454-460.
[12] 王波. 2D-LC在酒花颗粒的T、U-酸、异T-酸及蛋糕中合成色素检测的应用研究[D]. 兰州:甘肃农业大学, 2009.
[13] 蔡玮红, 冼燕萍,罗海英,等. UPLC-MS/MS法同时测定焙烤食品中的丙烯酰胺、4-甲基咪唑与5-羟甲基糠醛[J]. 现代食品科技, 2014, 30(2): 249-254.
[14] 孙建平. 葡萄与葡萄酒中酚类物质LC-UV-MS/MS谱库构建及应用[D]. 北京:中国农业大学, 2006.
[15] ASENSTORFER R E, LEE D F, JONES G P. Influence of structure on the ionisation constants of anthocyanin and anthocyanin-like wine pigments[J]. Analytica Chimica Acta, 2006, 563: 10-14.
[16] ROMERO C, BAKKER J. Anthocyanin and colour evolution during maturation of four port wines: Effect of pyruvic acid addition[J].Journal of the Science of Food and Agriculture, 2000, 81: 252-260.
[17] CAPRON X, SMEYERS-VERBEKE J, MASSART D L. Multivariate determination of the geographical origin of wines from four different countries[J]. Food Chemistry, 2007, 101: 1 585-1 597.
