Maltotetraose amylase hydrolyzes starch or maltodextrin to form maltotetraose. In order to reduce costs, the fermentation condition of previously constructed recombinant Bacillus subtilis that produces maltotetraose amylase was optimized by optimizing the nitrogen and carbon sources of the medium. It was found that when the medium contained 25 g/L soybean meal, 25 g/L industrial peptone, and 5 g/L glycerol, fermented at 33 ℃ and 200 r/min for 48 h and at a seeding rate of 5%, the activity of fermented maltotetraose amylase was up to 236 U/mL. Furthermore, maltotetraose was prepared with the enzyme and the reaction condition was optimized, and the products were detected by HPLC. The results showed that when the reaction temperature was 50 ℃, the reaction pH was 7.0, the amount of enzyme was 30 U/g substrate, the concentration of substrate (maltodextrin) was 250 g/L, and reacted for 12 h, the conversion rate of maltotetraose reached 73.2%. This study provides a theoretical basis for reducing production costs and preparing maltotetraose at an industrial scale.
YANG Yanan
,
SU Lingqia
,
WU Jing
. Optimized fermentation condition of recombinant Bacillus subtilis producing maltotetraose amylase and maltotetraose preparation[J]. Food and Fermentation Industries, 2019
, 45(8)
: 44
-49
.
DOI: 10.13995/j.cnki.11-1802/ts.018948
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