The strains that are capable to transform L-tyrosine to tyrosol were applied to a Chinese medicine Rhodiola to increase the contents of its active ingredients, such as tyrosol and salidroside, and to enhance its antioxidant activity. Tyrosine decarboxylase producing strains were screened using a tyrosine-containing plate through pH changes, and nine strains that could produce tyrosol were determined by high performance liquid chromatography (HPLC) and liquid chromatograph-mass spectrometry (LC-MS). Rhodiola was fermented by these strains, and the yield of tyrosol after fermentation was 0.78 g/L, which increased by 235%. The content of salidroside increased by 109% and reached 0.43 g/L. The total reducing power after fermentation was 1.7 times higher than that of the extract, and the DPPH scavenging capacity increased from 35.45% to 61.01%. This study provides a new idea for developing and applying Rhodiola in the future.
WU Jie
,
WU Chuanchao
,
GU Qiuya
,
YU Xiaobin
. High value conversion of effective components in Rhodiolaby microbial fermentation[J]. Food and Fermentation Industries, 2019
, 45(9)
: 29
-34
.
DOI: 10.13995/j.cnki.11-1802/ts.019415
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