Hemolysis tests, antibiotics susceptibility tests, and PCR amplification of virulence genes and drug resistance genes were used to assess the safety of Enterococcus faecium Ef2. The results of hemolysis tests were negative. E. faecium Ef2 was not sensitive to gentamicin and streptomycin, but it was sensitive to vancomycin and most of other antibiotics. No virulence genes were detected, and therefore the strain could be preliminarily determined as safe. The induction medium and culture conditions of producing amine oxidase by this strain were optimized. The optimal components of the induction medium included 50 g/L lactose, 6 g/L putrescine, 8 g/mL yeast extract, 3 g/L MgSO4, 0.03 g/L MnSO4, and 1.5 g/L sodium acetate. The optimal culture condition was as follows: initial pH of 7.0, 0.5% inoculation, cultured for 36 h at 32 ℃. The activity of amine oxidase increased by 139.63% to 26.12 U/mL after induction. The results showed that the activity of amine oxidase produced by E. faecium Ef2 could be greatly increased by optimizing the culture and induction conditions, which lays a foundation for further study on producing amine oxidase by this strain.
LI Binbin
,
SONG Guisen
,
QUAN Xinjie
,
PU Yan
,
XU Ye
,
NIU Shuhui
,
AO Xiaolin
,
CHEN Shujuan
,
HE Li
,
LIU Shuliang
,
YANG Yong
. Safety assessment and optimized cultural and induction conditions forEnterococcus faecium Ef2 producing amine oxidase[J]. Food and Fermentation Industries, 2019
, 45(9)
: 41
-48
.
DOI: 10.13995/j.cnki.11-1802/ts.019666
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