Effects of Bacillus amyloliquefaciens exopolysaccharides on proliferation and metabolism of lactic acid bacteria

  • CAI Guolin ,
  • LIU Yifan ,
  • LI Xiaomin ,
  • LU Jian
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  • 1(Key Laboratory of Industrial Biotechnology, Ministry of Education (Jiangnan University), Wuxi 214122, China)
    2(National Engineering Laboratory for Cereal Fermentation Technology (Jiangnan University), Wuxi 214122, China)
    3(School of Biotechnology,Jiangnan University, Wuxi 214122, China)

Received date: 2018-12-29

  Online published: 2019-06-17

Abstract

This study aimed to investigate the effects of exopolysaccharides from Bacillus amyloliquefaciens JN4 (EPS-JN4) on the propagation and metabolism of probiotics. The specific proliferative effects of EPS-JN4 were analyzed by PCR-denaturing gradient gel electrophoresis, and their effects on the growth, metabolites, intestinal tolerance, and surface hydrophobicity of lactic acid bacteria were also determined. It was found that EPS-JN4 had a specific proliferative effect on Lactobacillus reuteri. Compared to glucose, the early proliferation rate of L. reuteri JN125 treated with EPS-JN4 was slower, but its highest biomass was 4.25 times higher. In addition, the contents of lactic acid and acetic acid reduced by 12.2% and 32.4%, respectively. The intestinal tolerance and surface hydrophobicity of EPS-JN4-treated L. reuteri JN125 significantly improved, which could be due to the protein and lipid contents in cell membranes increased by 40.8% and 105.7%, respectively. Besides, the proportions of 18 C fatty acids and unsaturated fatty acids in cell membranes significantly increased. In conclusion, EPS-JN4 can be used as a potential prebiotic to regulate the intestinal health of animals.

Cite this article

CAI Guolin , LIU Yifan , LI Xiaomin , LU Jian . Effects of Bacillus amyloliquefaciens exopolysaccharides on proliferation and metabolism of lactic acid bacteria[J]. Food and Fermentation Industries, 2019 , 45(10) : 16 -21 . DOI: 10.13995/j.cnki.11-1802/ts.019814

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