Food and Fermentation Industries >

2019 , Vol. 45 >Issue 10: 22 - 28

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.019842

Mutagenesis and fermentation optimization of Escherichia coli K235 producing high molecular weight polysialic acid

  • GAO Lin ,
  • ZHU Li ,
  • YANG Zelin ,
  • ZHAN Xiaobei ,
  • WU Jianrong
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  • 1(School of Biotechnology, Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China)
    2(Wuxi Glyco Biotechology Co. Ltd, Wuxi 214125, China)

Received date: 2019-01-03

  Online published: 2019-06-17

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Abstract

In order to produce polysialic acid (PSA) with high molecular weight, Escherichia coli K235 was treated by atmospheric and room temperature plasma (ARTP) and diethyl sulfate (DES). Mutant 4B31 obtained by ARTP mutagenesis produced PSA with 36.84% higher molecular weight than that of the parent strain. Mutant 6E61 obtained from mutant 4B31 by DES mutagenesis produced PSA with 78.18% higher molecular weight. By using a three-stage agitation speed fermentation, the maximal molecular weight of PSA produced by mutant 6E61 was 430.5 kDa, which was the highest value reported up to date.

Key words： Escherichia coli; polysialic acid; high molecular weight; fermentation

Cite this article

GAO Lin , ZHU Li , YANG Zelin , ZHAN Xiaobei , WU Jianrong . Mutagenesis and fermentation optimization of Escherichia coli K235 producing high molecular weight polysialic acid[J]. Food and Fermentation Industries, 2019 , 45(10) : 22 -28 . DOI: 10.13995/j.cnki.11-1802/ts.019842

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