The fermentation condition for producing GlcUA by Zygosaccharomyces rouxii ZSR2, a GlcUA producer, was optimized by single factor experiments. It was found that the titer of GlcUA under the optimal fermentation condition (the medium contained 80 g/L sucrose, 30 g/L soy peptone, initial pH=5.0, with 3% inoculum and aged for 9 h) was 14.68 g/L, which was 3.8 times higher than that of unoptimized. Besides, the production of GlcUA enhanced to 22.36 g/L by fed-batch fermentation, which was the highest level reported regarding GlucUA production by single strain fermentation. In conclusion, this study lays a foundation for industrial production of GlcUA by microbial fermentation.
LI Yifeng
,
FANG Fang
. Optimized fermentation condition for Zygosaccharomyces rouxii producing glucuronic acid[J]. Food and Fermentation Industries, 2019
, 45(10)
: 42
-47
.
DOI: 10.13995/j.cnki.11-1802/ts.019905
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