The lactic acid bacteria (LAB) for wine making in China are mostly imported from foreign manufactures, which has caused serious homogenization of wine quality. In this study, wild-type LABs from natural malolactic fermented red wine in Gansu Hexi Corridor were isolated, identified and their fermentation adaptability was examined. Three strains that could initiate malolactic fermentation and degrade acids were identified as Pediococcus parvulus. Of which, strain C30 showed significantly higher biomass yield at low fermentation temperature (15℃), high ethanol concentration (14%) and high SO2 concentration (50 mg/L) than that of commercial LAB. In conclusion, strain C30 could be used as a malolactic fermentation starter for wines and has potential value in improving the wine quality in Gansu Hexi Corridor.
HAO Nan
,
MA Tengzhen
,
YUN Jianmin
,
YANG Xueshan
,
LI Aixia
,
CHEN Yanxiong
,
HAN Shunyu
. Isolation and brewing characteristics of wild-type lactic acid bacteria from Gansu Hexi Corridor[J]. Food and Fermentation Industries, 2019
, 45(10)
: 142
-148
.
DOI: 10.13995/j.cnki.11-1802/ts.019590
[1] ZAPPAROLI G, TOSI E, AZZOLINI M, et al. Bacterial inoculation strategies for the achievement of malolactic fermentation in high alcohol wines[J].South African Society for Enology & Viticulture, 2009, 30(1): 49-55.
[2] BARTOWSKY E, COSTELLO P, HENSCHKE P. Management of malolactic fermentation-wine flavor manipulation[J].Australian & New Zealand Grapegrower & Winemaker, 2002, 46(1): 10-12.
[3] BAUER R, DICKS L M T. Control of malolactic fermentation in wine. A review[J].South African Society for Enology & Viticulture, 2004,25(2): 74-88.
[4] BORDAS M, ARAQUE I, BORDONS A, et al. Differential expression of selected Oenococcus oeni genes for adaptation in wine-like media and red wine[J].Annals of Microbiology, 2015, 65(4):2 277-2 285.
[5] CINQUANTA L, DE STEFANO G, FORMATO D, et al. Effect of pH on malolactic fermentation in southern Italian wines[J]. European Food Research and Technology, 2018, 244(7):1 261-1 268.
[6] 乔慧,卢柯,杨世玲,等.中国主要葡萄酒产区酒酒球菌糖苷酶活性[J].食品科学,2014,35(23):144-150.
[7] 苏静.高抗氧化活性酒酒球菌的筛选及提升葡萄酒品质的研究[D].杨凌:西北农林科技大学,2016.
[8] MESAS J M, RODRIGUEZ M C, ALEGRE M T. Basic characterization and partial purification of β-glucosidase from cell-free extracts of Oenococcus oeni ST81[J]. Applied Microbiology, 2012, 55(3):247-255.
[9] SICO M A, BONOMO M G, SALZSNO G. Isolation and characterization of Oenococcus oeni from Aglianico wines[J].World Journal of Microbiology and Biotechnology, 2008, 24 (9): 1 829-1 835.
[10] CRUZPIO L E, POVEDA M, ALBERTO M R, et al. Exploring the biodiversity of two groups of Oenococcus oeni isolated from grape musts and wines: Are they equally diverse?[J]. Systematic & Applied Microbiology, 2017, 40(1):1-10.
[11] ROMERO J, ILABACA C, RUIZ M, et al. Oenococcus oeni in chilean red wines:Technological and genomic characterization[J]. Frontiers in Microbiology, 2018, 9:1-12.
[12] RODRÍGUEZ-NOGALES J M, VILA-CRESPO J, FERNÁ-NDEZ-FERNÁNDEZ E. Immobilization of Oenococcus oeni in lentikats to develop malolactic fermentation in wines[J]. Biotechnology Progress, 2013, 29(1):60-65.
[13] BORDAS, M, ARAQUE I, ALEGRET J O, et al. Isolation, selection, and characterization of highly ethanol-tolerant strains of Oenococcus oeni from south Catalonia[J]. International Microbiology the Official Journal of the Spanish Society for Microbiology, 2013, 16(2):113-123.
[14] JIANG J, SUMBY K M, SUNDSTROM J F, et al. Directed evolution of Oenococcus oeni strains for more efficient malolactic fermentation in a multi-stressor wine environment[J]. Food Microbiology, 2018, 73:150-159.
[15] JUEGA M, COSTANTINI A, BONELLO F, et al. Effect of malolactic fermentation by Pediococcus damnosus on the composition and sensory profile of Albariño and Caiño white wines[J]. Journal of Applied Microbiology, 2014, 116(3):586-595.
[16] 李平兰,贺稚非.食品微生物学实验原理与技术[M].北京:中国农业出版社,2014.
[17] 任晓宁,张宇,陈其玲,等.不同发酵条件对模拟葡萄酒中酒酒球菌柠檬酸代谢的影响[J].食品工业科技,2017,38(4):180-185.
[18] 钱存柔,黄仪秀.微生物学实验教程[M].北京:北京大学出版社,1999.
[19] 凌代文.乳酸细菌分类鉴定及实验方法[M].北京:中国轻工业出版社,1999.
[20] BUCHANAN R E.伯杰细菌鉴定手册.中国科学院微生物研究所,译[M].北京:科学出版社,1984.
[21] HENICKLING T, SANDINE W E, HEATHRBELL D A. Evaluation of malolactic bacteria isolated from Oregon wine[J]. Appl Environ Microbiol, 1989, 55(8): 2 010-2 016.
[22] OTIENO D O, ASTHTON J F, SHAH N P. Isoflavone phytoestrogen degradation in fermented soymilk with selected β-glucosidase producing L. acidophilus strains during storage at different temperatures[J]. International Journal of Food Microbiology, 2007, 115(1):79-88.
[23] DICKS L M T, DELLAGLIO F, COLLINS M D. Proposal to rechssify Leuconostoc oenos as Oenococcus oeni[J]. Bacteriol, 1995, 45(2): 395-397.
[24] WU Chongde, HUANG Jun, ZHOU Rongqing. Progress in engineering acid stress resistance of lactic acid bacteria[J]. Applied Microbiology and Biotechnology, 2014, 98(3):1 055-1 063.
[25] BLATTEL V, LARISIKA M, PFEIFFER P, et al. β-1,3-glucanase from Delftia tsuruhatensis strain MV01 and its potential application in vinification[J]. Applied & Environmental Microbiology, 2011, 77(3):983-990.
[26] BETTERIDGE A L, SUMBY K M, SUNDSTROM J F, et al. Application of directed evolution to develop ethanol tolerant Oenococcus oeni for more efficient malolactic fermentation[J]. Appl Microbiol Biotechnol, 2018, 102(2):921-932.