Diacylglycerols isomers (1,3-/1,2-DAGs) play an important role in identifying olive oil quality. The ratio of DAGs isomers can indicate the freshness of olive oil. Solid phase extraction was used to extract DAGs in oil, and they were quantified by GC-FID after derivatization. Derived at 30 ℃ for 1 h was the optimal derivative condition. The lowest detection limit of this method was 0.032 mg/mL, the RSD value was 1.35%-4.62%, and the average recovery rate of each DAG was 73.9%-76.9%. It was found that the ratios of DAGs isomers in six extra-virgin olive oil and four refined olive oil were significantly different. This method was simple and easy to operate. It can be used to accurately determine DAGs isomers in olive oil. It provides technical supports for developing olive oil authenticity detection methods as well as establishing product standards. It also has guiding significance for portal and market regulations on imported extract virgin olive oil.
WEI Yao
,
ZHONG Qiding
,
WANG Daobing
,
LI Guohui
. Detection method of diacylglycerols isomers in olive oil and its application[J]. Food and Fermentation Industries, 2019
, 45(10)
: 201
-207
.
DOI: 10.13995/j.cnki.11-1802/ts.018832
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