Isolation, purification and antioxidant activities of extracellular polysaccharidesfrom Rhodotorula mucilaginosa

  • MA Wenjin ,
  • LI Meilin ,
  • WANG Bo ,
  • ZHANG Yongxian ,
  • YU Changqing ,
  • BAN Shenghua
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  • (Gansu Institute of Light Industry Limited Liability Company, Lanzhou 730030, China)

Received date: 2019-01-07

  Online published: 2019-07-08

Abstract

The primary structures and antioxidant activities of Rhodotorula mucilaginosa extracellular polysaccharides were studied in this paper. A single component REPS2-A in extracellular polysaccharides was obtained by isolation and purification, followed by measuring its molecular weight distribution and free radical scavenging activities. The results showed that the molecular weight of REPS2-A was 7.125×106 Da. Moreover, the scavenging rates of DPPH and ABTS free radicals by 8 mg/mL REPS2-A were 49.1% and 51.2%, respectively, and the reducing power was 0.352. This paper provides a new data basis for understanding the structures and functions of extracellular polysaccharides of R. mucilaginosa, which provides an experimental basis for subsequent studies on correlations between their structures and activities.

Cite this article

MA Wenjin , LI Meilin , WANG Bo , ZHANG Yongxian , YU Changqing , BAN Shenghua . Isolation, purification and antioxidant activities of extracellular polysaccharidesfrom Rhodotorula mucilaginosa[J]. Food and Fermentation Industries, 2019 , 45(11) : 65 -70 . DOI: 10.13995/j.cnki.11-1802/ts.019872

References

[1] LIU S B, QIAO L P, HE H L, et al. Optimization of fermentation conditions and rheological properties of exopolysaccharide produced by deep-sea bacterium Zunongwangia profunda SM-A87[J]. PLoS One, 2011, 6(11): 1-11.
[2] GRIJPSTRA J,GERWIG G J,WÖSTEN H, et al. Production of extracellular polysaccharides by CAP mutants of Cryptococcus neoformans[J]. Eukaryotic Cell, 2009, 8(8): 1 165-1 173.
[3] PAROLIS L A S, DUUS J, PAROLIS H, et al. The extracellular polysaccharide of Pichia (Hansenula) holstii NRRL Y-2448:The structure of the phosphomannan backbone[J]. Carbohydrate Research, 1996, 293(1): 101-117.
[4] NISHIMURA K, SHIMADA K, IWASAWA H, et al. Total DNA preparation excluding extracellular acidic polysaccharide from\r, Lipomyces\r, yeasts and its application to taxonomic studies[J]. Bioscience, Biotechnology and Biochemistry, 2002, 66(7):1 563-1 566.
[5] LIM J M, LEE Y J, CHO H R, et al. Extracellular polysaccharides purified from Aureobasidium pullulans SM-2001 (Polycan) inhibit dexamethasone-induced muscle atrophy in mice[J]. International Journal of Molecular Medicine, 2018, 41(3): 1 245-1 264.
[6] 康雨芳, 陈雪峰,常相娜,等. 胶红酵母胞外多糖的抗疲劳作用[J]. 食品工业科技, 2018,39(9): 301-305.
[7] JIN L G, YOU J C, GUO Q S, et al. Study on phenol-sulfuric acid method for determination of polysaccharide content in Pleurotus eryngii[J]. Food Science, 2008, 29(12): 555-558.
[8] CHI Z, ZHAO S. Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain[J]. Enzyme and Microbial Technology, 2003, 33(2): 206-211.
[9] PENG Q, LYU X, XU Q, et al. Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum[J]. Carbohydrate Polymers, 2012, 90(1): 95-101.
[10] LI C, FU X, LUO F, et al. Effects of maltose on stability and rheological properties of orange oil-in-water emulsion formed by OSA modified starch[J].Food Hydrocolloids,2013,32(1):79-86.
[11] WANG R, CHEN P, JIA F, et al. Characterization and antioxidant activities of polysaccharides from Panax japonicus C.A. Meyer[J]. Carbohydrate Polymers, 2012, 88(4): 1 402-1 406.
[12] ZHU K, ZHOU H, QIAN H. Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase[J]. Process Biochemistry, 2006, 41(6): 1 296-302.
[13] CHENG H, FENG S, JIA X, et al. Structural characterization and antioxidant activities of polysaccharides extracted from Epimedium acuminatum[J]. Carbohydrate Polymers, 2013, 92(1): 63-68.
[14] LI Q, YU N, WANG Y, et al. Extraction optimization of Bruguiera gymnorrhiza polysaccharides with radical scavenging activities[J]. Carbohydrate Polymers, 2013, 96(1): 148-155.
[15] 景永帅, 吴兰芳,王乾,等. 远志多糖提取工艺优化及其抗氧化活性研究[J]. 食品与机械, 2016,32(5): 152-156.
[16] GHOSH P K, SARKAR A, PRAMANIK K, et al. The extracellular polysaccharide produced by Enterobacter spp. isolated from root nodules of Abrus precatorius L.[J]. Biocatalysis and Agricultural Biotechnology, 2016, 5:24-29.
[17] 李彬, 陈向楠,张建法,等. 产胞外多糖菌株的筛选及胞外多糖结构分析[J]. 生物技术通报, 2016, 32(5): 165-171.
[18] LIANG L, WU X, ZHU M, et al. Chemical composition, nutritional value, and antioxidant activities of eight mulberry cultivars from China[J]. Pharmacognosy Magazine, 2012, 8(31): 215-224.
[19] ZHANG A Q, XIAO N N, DENG Y L, et al. Purification and structural investigation of a water-soluble polysaccharide from Flammulina velutipes[J]. Carbohydrate Polymers, 2012, 87(3): 2 279-2 283.
[20] IRSHAD M, ZAFARYAB M, SINGH M, et al. Comparative analysis of the antioxidant activity of Cassia fistula extracts[J]. International Journal of Medicinal Chemistry, 2012, 2012: 1-6.
[21] 潘乔丹, 黄元河,唐海燕,等. 赤苍藤和密蒙花多糖的含量测定及抗氧化研究[J]. 食品研究与开发, 2016,37(22): 6-9.
[22] ZOU C, DU Y, LI Y, et al. Preparation of lacquer polysaccharide sulfates and their antioxidant activity in vitro[J]. Carbohydrate Polymers, 2008, 73(2): 322-331.
[23] HU C, ZHANG Y, KITTS D D. Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var. Henonis leaf extract in vitro[J]. Journal of Agricultural & Food Chemistry, 2000, 48(8): 3 170-3 176.
[24] WANG Z, ZHAO Y, SU T, et al. Characterization and antioxidant activity in vitro and in vivo of polysaccharide purified from Rana chensinensis skin[J]. Carbohydrate Polymers, 2015, 126: 17-22.
[25] 陈欣, 龚兰,刘冠卉. 食用真菌多糖提取条件的优化及其还原力的比较[J]. 食品科学, 2010, 31(14): 140-144.
[26] CABIB E, SILVERMAN S J, SHAW J A, et al. Cheminform abstract: Carbohydrates as structural constituents of yeast cell wall and septum[J]. Cheminform, 1991, 22(39): 483-489.
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