Antioxidant activities of enzymatic hydrolysates of Meretrix meretrix L. with different molecular weights were compared, and the enzymatic hydrolysate components with low molecular weight (LMEC) were analyzed. A stable double-enzyme hydrolysis technique with flavor protease and neutral protease to prepare Meretrix meretrix L. hydrolysates was established. The LMEC presented relatively good antioxidant activities, as their total reducing power was 54.16%, and its maximum scavenging rates of hydroxyl radicals, DPPH free radicals and superoxide anions were 53.5%, 49%, and 47.7%, respectively. Besides, LMEC included 79% oligo-peptides with an average molecular weight of 1.05 kDa, and it was rich in Ala, Thr, Val, Leu, Pro, and Glu. According to UPLC-MS analysis, LMEC were speculated to have six possible peptides. This study provides a theoretical basis for understanding the antioxidant activity of Meretrix meretrix L. and also for separating active peptides in the future.
HU Dawei
,
LI Heng
,
JIANG Min
,
DING Hailong
,
WANG Songtao
,
SHEN Caihong
,
XU Zhenghong
,
SHI Jinsong
. Antioxidant evaluation and composition characterization of enzymatic hydrolysates of Meretrix meretrix L.[J]. Food and Fermentation Industries, 2019
, 45(12)
: 207
-214
.
DOI: 10.13995/j.cnki.11-1802/ts.020295
[1] 赵莎莎,赵玉勤,张亚茹,等.文蛤多肽的制备及对非酒精性脂肪肝细胞模型的修复作用[J].中国食品学报, 2017,17(6):33-39.
[2] 何耀涛,邓家刚,杜成智,等.文蛤肉药用价值研究概况[J].广西中医药大学学报,2017,20(3):65-67.
[3] YIN Hua,YUAN Qiang, CHU Yunyue. Extraction and determination of polysaccharides from Meretrix meretrix Linnaeus[J].Marine Drugs,2006,25(1):48-53.
[4] HSIEH Y L,TENG H J,YEH Y H,et al.The protective effects of clams on hypercholesterolemia in late-stage triple-transgenic Alzheimer's diseased mice hearts[J].Marine Drugs,2018,16(8):263.
[5] HUANG Fangfang,ZHAO Shasha,YU Fangmiao,et al.Protective effects and mechanism of Meretrix meretrix oligopeptides against nonalcoholic fatty liver disease[J].Marine Drugs,2017,15(2):31-44.
[6] 严晓丹,钱建瑛,许泓瑜,等.文蛤不同极性提取物对糖尿病小鼠降血糖作用的研究[J].中国海洋药物,2015,34(5):71-76.
[7] 潘静,徐惠,张小蒙,等.文蛤活性肽的研究现状及应用前景[J].科技资讯,2017,15(10):239-241.
[8] 甘瑾.玛咖(Lepidium meyenii Walp.)抗氧化活性及活性物质基础的研究[D].北京:北京林业科学研究院,2013:1-112.
[9] UMAYAPARVATHI S,MEENAKSHI S,VIMALRAJ V,et al.Antioxidant activity and anticancer effect of bioactive peptide from enzymatic hydrolysate of oyster(Saccostrea cucullata)[J].Biomedicine and Nutrition,2014(4):343-353.
[10] CHEN Ling,HUANG Gangliang.Antioxidant activities of phosphorylated pumpkin polysaccharide[J]. International Journal of Biological Macromolecules,2019,125:256-261.
[11] 杨晋,陶宁萍,王锡昌.文蛤的营养成分分析及其用于海味香精的酶解液制备[J].食品工业科技,2007,4:146-149.
[12] WANG Lingchong,CHEN Liye,LI Junsong.Structural elucidation and immune-enhancing activity of peculiar polysaccharides fractioned from marine clam Meretrix meretrix (Linnaeus)[J].Carbohydrate Polymers,2018,201:500-513.
[13] 李和生,刘智勇,王鸿飞.文蛤多肽组分的分离及其抗氧化活性研究[J].中国食品学报,2012,12(6):30-34.
[14] 綦蕾,王振宇.松仁多肽的抗氧化活性研究[J].食品科学,2010,35(10):110-113.
[15] 孟娟霞,赵明峰.活性氧与组织器官纤维化[J].中国实验血液学杂志,2012,20(5):1 284-1 288.
[16] 刘占才,牛俊英.超氧阴离子自由基对生物体作用机理的研究[J].焦作教育学院学报,2002,18(4):48-51.
[17] LIANG Mingcai,WANG Zhengxuan,LI Hui,et al.L-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway[J].Food and Chemical Toxicology,2018,115:315-328.
[18] NAZEER R A,PRABHA K R D, KUMAR N S S,et al.Isolation of antioxidant peptides from clam Meretrix casta (Chemnitz)[J].Food Sci Technol,2013,50(4):777-783.
[19] 张泽,赵前程,程继龙,等.长牡蛎蛋白双酶水解工艺及其产物的抗氧化活性[J].湖南农业科学,2012,52(11):2 315-2 317.
[20] 张昊,任发政.天然抗氧化肽的研究进展[J].食品科学,2008,29(4):443-447.
[21] JET L L,MILLER R H.Amino acid metabolism during exercise in trained rats: The potential role of carnitine in the metabolism of branched chain amino acid[J].Metabolism,1987,36(8):748-752.
[22] MOURA C S,LOLLO P C B,MORATO P N,et al.Modulatory effects of arginine, glutamine and branched-chain amino acids on heat shock proteins, immunity and antioxidant response in exercised rats[J].Food & Function,2017,8(9):3 228-3 238.
[23] CHAI T T,LAW Y C,WONG F C.Enzyme-assisted discovery of antioxidant peptides from edible marine invertebrates:A review[J].Marine drugs,2017,15(2):42-58.
[24] 张晖,唐文婷,王立,等.抗氧化肽的构效关系研究进展[J].食品与生物技术学报,2013,32(7):673-679.
[25] WU Yanyan,WANG Jing,LI Laihao,et al.Purification and identification of an antioxidant peptide from Pinctada fucata muscle [J].Journal of Food,2018,1(16):11-19.
[26] JIN J E,AHN C B,JE J Y.Purification and characterization of antioxidant peptides from enzymatically hydrolyzed ark shell (Scapharca subcrenata)[J].Process Biochemistry,2018,72:170-176.
[27] WANG Xueqin,YU Huahua,XING Rongen,et al.Purification and identification of antioxidative peptides from mackerel (Pneumatophorus japonicus) protein[J].RSC Advances,2018,8(37):20 488-20 498.
