Simultaneous determination of antioxidants and p-hydroxybenzoate antiseptics in yeast products using QuEChERS and high-performance liquid chromatography-tandem mass spectrometry

  • LI Yanmei ,
  • LI Fang ,
  • SU Youzhi ,
  • ZHOU Jun ,
  • LUO Qiong ,
  • WANG Jingwei ,
  • WANG Xinglei
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  • 1. Key Laboratory of Pollutant Chemistry and Environmental Treatment, YiLi Normal University, Yining 835000, China;
    2. Technical Centre of Yining Customs, Yining 835000, China

Received date: 2018-11-30

  Revised date: 2019-03-27

  Online published: 2019-07-16

Abstract

A qualitative and quantitative method based on high performance liquid chromatography-tandem mass spectrometry for simultaneous determination of four antioxidants and five p-hydroxybenzoate antiseptics in yeast products was developed. Samples were defatted and extracted with acetonitrile-hexane mixture, and then concentrated to dry and redissolved in the mobile phase. The solution was cleaned by C18 powder adsorption and filtered. There were nine analytes determined by using electrospray ion source in multiple reaction monitoring mode. The results showed that all analytes had good linearity in the range of 0.1-10 mg/L with correlation coefficients (r2) larger than 0.992, and the quantitation limits of the method were in the range of 0.10-0.50 mg/kg, and the average recovery rate was 78.9%-108.6%, and RSDs were 4.2%-15.8%. This convenient and accurate method provides technical supports for detecting such substances based on high throughput technology.

Cite this article

LI Yanmei , LI Fang , SU Youzhi , ZHOU Jun , LUO Qiong , WANG Jingwei , WANG Xinglei . Simultaneous determination of antioxidants and p-hydroxybenzoate antiseptics in yeast products using QuEChERS and high-performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2019 , 45(12) : 215 -221 . DOI: 10.13995/j.cnki.11-1802/ts.019494

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