Linear malto-oligosaccharides can be produced from corn starches by malto-oligosaccharides-forming amylase (Bst-MFAse). This study aimed to evaluate the effects of initial substrate concentration on producing linear malto-oligosaccharides by Bst-MFAse using the contents and yields of linear malto-oligosaccharides as indicators, followed by analyzing related mechanisms. The results showed that when the initial concentration of corn starch increased from 10% to 50% (w/w), the contents of linear malto-oligosaccharides and main products (maltopentaose and maltohexaose) increased gradually after hydrolyzing for 24 h. Besides, their contents reached the highest with 45% substrate, which were 344.47 and 192.33 mg/g, respectively. However, the yield of linear malto-oligosaccharides decreased gradually. This might be due to the increased substrate concentration enhanced the viscosity of the system during liquefaction and saccharification, which resulted in decreased water molecule mobility and significantly reduced free water amount. Additionally, MFAse had different action modes among different concentrations of corn starches, which could cause insufficient hydrolysis of corn starch molecules at high concentration. Overall, this study provides a reference for enzymatic preparation of linear malto-oligosaccharides in industries.
CHEN Dianning
,
LI Caiming
,
GU Zhengbiao
,
HONG Yan
,
CHENG Li
,
LI Zhaofeng
. Effects of corn starch concentration on enzymatic preparation of linear malto-oligosaccharides[J]. Food and Fermentation Industries, 2019
, 45(13)
: 15
-22
.
DOI: 10.13995/j.cnki.11-1802/ts.020364
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