A rapid and sensitive qualitative and quantitative analysis method for hemin by ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) was established. The electrospray ion source was used for studying hemin at mass spectrometry multiple reaction monitoring positive ion mode. The characteristic ion pair with the strongest hemin signal was 616→557, and there was a good linear relationship in the range of 2.5-100 mg/L. The detection and quantitation limits of the method were 1.25 μg/L and 4.0 μg/L, respectively. The recovery rate ranged from 80.65% to 102.99%, and the precision was 3.84%. The results show that the method has high recovery rate and good precision, and therefore can be effectively used for quantitative and qualitative analysis of hemin in health care products.
LI Weili
,
WU Yao
,
LIU Yushu
,
YU Hui
,
REN Yanjiao
,
ZHOU Wenhua
,
WU Tao
. Rapid determination of hemin in foods by ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry[J]. Food and Fermentation Industries, 2019
, 45(13)
: 212
-216
.
DOI: 10.13995/j.cnki.11-1802/ts.019293
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