The methods to extract proteins from honey from different floral origins in China and their protein compositions were compared. Jujube honey, chaste honey, lungan honey, Apis cerana honey, acacia honey, and rape honey were selected to compare their differences in protein electrophoresis. Besides, the feasibilities and effects of applying ultrafiltration and sulfuric acid-sodium tungstate precipitation in extracting proteins were analyzed. The relative molecular weights of proteins from the six floral honey were in the range of 14.4-97.4 kDa, but their protein compositions and contents were significantly different. In addition, Jujube honey, lungan honey, and Apis cerana honey contained more high-molecular weight proteins (>97.4 kDa). Moreover, proteins from jujube honey, chaste honey, acacia honey, and rape honey were better extracted by ultrafiltration, while sulfuric acid-sodium tungstate precipitation method was suitable for extracting proteins from lungan and Apis cerana honey. Furthermore, the proteins obtained by these two methods showed clearly different molecular weights and hydrophobic characteristics. Therefore, this study provides a theoretical foundation for identifying floral origin of honey based on protein differences.
ZHANG Ying
,
ZHANG Guangyan
,
WANG Yuxiang
,
CAO Wei
. Comparison of protein composition and extraction methods between honey from different floral origins[J]. Food and Fermentation Industries, 2019
, 45(14)
: 91
-96
.
DOI: 10.13995/j.cnki.11-1802/ts.019746
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