A direct analysis in real-time of atmospheric pressure ion source serial mass spectrometry (DART-MS) method was established to rapidly determine the contents of capsaicinoids (capsaicin and dihydrocapsaicin) in various capsicum and its products. The pyrolysis behaviors of capsaicin and dihydrocapsaicin by the DART ion source were systematically studied, followed by optimizing gas desorption temperature and grid electrode voltage of DART ion source. The results showed that 0.50-50.0 μg/mL capsaicin and dihydrocapsaicin had good linear relationships under optimal experimental conditions. The detection limits and quantitation limits of the method were 0.1 μg/g and 0.5 μg/g, respectively. The recovery rates of capsaicin and dihydrocapsaicin were 79.74%-97.74% and 85.60%-98.71%, respectively, and precisions were 0.80%-8.89% and 2.69%-9.54%, respectively. The method was rapid, easy to operate, accurate and reliable, and therefore can be used for rapid evaluation of capsaicinoids.
LI Weili
,
LIU Yushu
,
WU Yao
,
YU Hui
,
REN Yanjiao
,
ZHOU Wenhua
,
CHE Zhenming
,
WU Tao
. Rapid real-time determination of capsaicinoids in capsicum and its products based on DART-MS/MS[J]. Food and Fermentation Industries, 2019
, 45(15)
: 273
-278
.
DOI: 10.13995/j.cnki.11-1802/ts.019624
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