The most effective metal ion was selected and the optimal culture condition was determined for RS66CD biofilm formation. The tolerance of biofilm to temperature, pH, salt concentration and bile salt was also studied. After adding Na+, Mn2+, Ca2+, Mg2+ and Fe3+, biofilm formation was determined by the 96-well method and SEM and then the best metal ion was selected. Under different ion concentrations, growth temperatures, and growth times, the biofilm formation was also studied to identify the optimal condition. The result showed that NaCl concentration of 53 g/L, the culture time of 24 h, and the culture temperature of 40 ℃ were the optimal condition for RS66CD biofilm formation. The environmental tolerance of strains after biofilm formation was improved. Na+ can promote the biofilm formation of Lactobacillus plantarum. In industrial production, microorganisms can be kept active by biofilms under stress condition.
ZHAO Jiawei
,
AO Xiaolin
,
CAI Yimin
,
LIU Shuliang
,
CHEN Anjun
,
WAN Hu
,
XU Fei
,
WANG Fan
,
HE Jinyang
. Effect of metal ions on the biofilm formation and environmental tolerance ofLactobacillus plantarum RS66CD[J]. Food and Fermentation Industries, 2019
, 45(21)
: 46
-52
.
DOI: 10.13995/j.cnki.11-1802/ts.021544
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