At present, the main international researches of carotenoids in citrus focus on orange juice, and lack of study on the detection and difference analysis of carotenoids in different kinds of loose-skin mandarins. The extraction conditions of carotenoids in citrus were optimized, followed by rapid analysis by ultra-performance liquid chromatography (UPLC). The varieties and contents of carotenoid compounds in pulps and peels were compared and discussed. The results showed that â-cryptoxanthin was the major carotenoid of the tested loose-skin mandarins, with total average content of 8.44 mg/kg FW in pulps and 23.73 mg/kg FW in peels. There was a notable difference of carotenoid contents and composition between 34 varieties, with the average content in pulps descending as follows: tangerines (14.73 mg/kg FW)>mandarins (13.51 mg/kg FW)>hybrids (8.11 mg/kg FW), while the order in peels was tangerines (47.94 mg/kg FW)>hybrids (28.71mg/kg FW)>mandarins (21.80 mg/kg FW). It is significant to make full use of the functional components in loose-skin mandarins.
CHEN Xiyu
,
JIAO Bining
,
ZHANG Yaohai
. Determination and difference study of carotenoid compounds in major varietiesof loose-skin mandarins in China[J]. Food and Fermentation Industries, 2019
, 45(21)
: 250
-257
.
DOI: 10.13995/j.cnki.11-1802/ts.020161
[1] WU Houjiu. The situation and outlook of citrus processing industry in China[C]. China/FAO Citrus Symposium. Beijing,2001.
[2] 张静,朱丽莎,张耀海,等.不同晚熟柑橘中酚类物质的含量检测及分析[J].食品与机械,2018,34(12):27-33;69.
[3] 于玉涵.柠檬中黄酮类化合物和辛弗林的检测和含量特征分析[D].重庆:西南大学,2011.
[4] 冉玥.基于柑桔功能成份指纹图谱地理标志产品鉴别技术研究[D].重庆:西南大学,2013.
[5] 郑洁.我国不同品种金柑主要营养及活性成分研究[D].重庆:西南大学,2015.
[6] 龚蕾.不同柚类中酚类物质的检测及差异性研究[D].重庆:西南大学,2016.
[7] 陶俊,张上隆,徐建国,等.柑橘果实主要类胡萝卜素成分及含量分析[J].中国农业科学,2003,36(10):1 202-1 208.
[8] ZHANG Yaohai, JIAO Bining. Simultaneous determination of six protoalkaloids in Chinese local varieties of loose-skin mandarins and sweet oranges by strong cation exchange-high performance liquid chromatography[J]. Food Analytical Methods,2019, 12(3):677-686.
[9] 王磊.柑桔汁中肌醇和可溶性糖检测及含量研究[D].重庆:西南大学,2010.
[10] 何朝飞.柑橘地理标志产品挥发性成分及其产地溯源研究[D].重庆:西南大学,2013.
[11] 陶俊,张上隆.园艺植物类胡萝卜素的代谢及其调节[J].浙江大学学报,2003,29(5):585-590.
[12] MILANI A, BASJRNEJAD M, SHAHBAZI S, et al. Carotenoids: biochemistry, pharmacology and treatment[J]. British Journal of Pharmacology,2017,174(11):1 290-1 324.
[13] 胡建中,王可兴,潘思轶.高效液相色谱法测定柑橘汁中类胡萝卜素[J].食品科学,2006,27(12):634-636.
[14] ATTILA A, NAGY V, ZOLTAN S, et al. Comparative study on the carotenoid composition of the peel and the pulp of different citrus species[J]. Innovative Food Science and Emerging Technologies,2007,8(3):390-394.
[15] 李文爽,夏先春,何中虎.普通小麦类胡萝卜素组分的超高效液相色谱分离方法[J].作物学报,2016,42(5):706-713.
[16] JANE N E, PIA L M, LARS O D, et al. Optimized, fast-throughput UHPLC-DAD based method for carotenoid quantification in spinach, serum, chylomicrons and feces[J]. J Agric Food Chem,2017,65:973-980.
[17] JOSE A F, JORGE A M P, PRISCILLA P F, et al. Ultrasound-assisted liquid-liquid extraction followed by ultrahigh pressure liquid chromatography for the quantification of major carotenoids in tomato[J]. J Food Com Anal,2017,57:87-93.
[18] MATSUMOTO H, IKOMA Y, KATO M, et al. Quantification of carotenoids in citrus fruit by LC-MS and comparison of patterns of seasonal changes for carotenoids among citrus varieties[J]. J Agric Food Chem,2007,55(6):2 356-2 368.
[19] 赵大球,周华.植物类胡萝卜素分析方法研究进展[J].北方园艺,2011(2):195-199.
[20] AARTI S, SAYEED A, ANEES A. Green extraction methods and environmental applications of carotenoids-a review[J].RSC Adv.,2015(5):62 358-62 393.
[21] RODRIGO M J, CILLA A, BARBERA R, et al. Carotenoid bioaccessibility in pulp and fresh juice from carotenoid-rich sweet oranges and mandarins[J]. Food & Function,2015,6(6):1 950-1 959.
[22] GIUFFRIDA D, DUGO P, SALVO A, et al. Free carotenoid and carotenoid ester composition in native, orange juices of different varieties[J]. Fruits,2010,65(5):277-284.
[23] GB/T 8855—2008,新鲜水果和蔬菜-取样方法[S].北京:中国标准出版社,2008.
[24] HYOUNG S L, WILLIAM S C. Seasonal changes of carotenoid pigments and color in hamlin, earlygold and budd blood orange juices[J]. J Agric Food Chem,2001,49:877-882.
[25] 王彬,林亮,陈敏氡,等.南瓜类胡萝卜素含量的超高效液相色谱分析[J].农学学报,2017, 7(12):22-27.
