Ultrasonic-assisted extraction of total alkaloid from Moringa oleifera was optimized to improve its medicinal value. The inhibitory activity of the alkaloid on α-glucosidase was determined by in vitro experiment. Under optimum extraction conditions: 250 W of ultrasonic power, 30 min of extraction time and 1∶9 of solid-liquid ratio, the extraction rate of alkaloids was 32.78 mg/g, which was increased by about 33%. The alkaloid extracted from M. oleifera exhibited inhibiting activities on α-glucosidase in a dose- and time- dependent manner with half-inhibitory concentration (IC50) of 34 mg/L, while 86.11% inhibition was obtained at 120 mg/L. The inhibition was reversible and competitive. The results lay a foundation for the development of M. oleifera alkaloid as a natural source of α- glucosidase inhibitor.
JIANG Cuicui
,
LI Ying
,
QIU Songshan
,
ZHOU Yingbiao
. Ultrasonic-assisted extraction of alkaloids from Moringa oleiferaand its inhibitory activity on α-glucosidase[J]. Food and Fermentation Industries, 2019
, 45(22)
: 97
-102
.
DOI: 10.13995/j.cnki.11-1802/ts.021463
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