Effects of pH and specific growth rates on ε-poly-L-lysine biosynthesis in Streptomyces albulus

  • WANG Kaifang ,
  • PAN Long ,
  • DIAO Wenjiao ,
  • CHEN Xusheng ,
  • MAO Zhonggui
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  • (The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University,School of Biotechnology, Wuxi 214122, China)

Received date: 2019-01-30

  Online published: 2020-02-11

Abstract

ε-Poly-L-lysine (ε-PL) is an antimicrobial peptide secreted by Streptomyces albulus and is mainly used as a biological food preservative. In order to further understand factors that affecting ε-PL production efficiency, different pH values and specific growth rates of S. albulus M-Z18 on the biosynthesis of ε-PL were investigated in this study. The results showed that the cell yield (YX/S) gradually reduced with the decrease of pH (D=0.04 h-1), while the specific glucose uptake rate (qS), specific ε-PL formation rate (qP), and intracellular ATP concentration gradually increased. Similarly, with the increase of specific growth rate (pH 4.0), qS, qP, and intracellular ATP concentration increased gradually. Therefore, both low pH environment and high specific cell growth rate contributed to efficient accumulation of ε-PL. These results provide a new insight for understanding why S. albulus accumulated ε-PL in low pH environment, and also provide a guidance for subsequent bioprocess optimization to increase ε-PL production.

Cite this article

WANG Kaifang , PAN Long , DIAO Wenjiao , CHEN Xusheng , MAO Zhonggui . Effects of pH and specific growth rates on ε-poly-L-lysine biosynthesis in Streptomyces albulus[J]. Food and Fermentation Industries, 2019 , 45(23) : 8 -14 . DOI: 10.13995/j.cnki.11-1802/ts.020132

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