Food and Fermentation Industries >

2019 , Vol. 45 >Issue 22: 20 - 26

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.021066

Comparison of the alleviation effects of different Lactobacilli on chronic alcohol-induced liver injury

  • ZHU Shiya ,
  • ZHAI Qixiao ,
  • ZHAO Xing ,
  • SUN Xinkai ,
  • LU Jianghao ,
  • LI Huawen ,
  • ZHAO Jianxin ,
  • ZHANG Hao ,
  • TIAN Fengwei ,
  • CHEN Wei
Expand
  • 1 (State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2 (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    3 (Hebei Inatural Biological Technlogy Co.Ltd., Shijiazhuang 050800, China)

Received date: 2019-05-11

  Online published: 2020-02-16

Fold

Abstract

In this study, the alleviation effects of different Lactobacillus strains on chronic alcohol-induced liver injury in mice were investigated. C57BL/6 mice were randomly divided into control group, alcohol model group, positive control group and Lactobacillus strains intervention groups. After treatment for 8 weeks, serum enzymes,liver oxidative stress,serum endotoxin levels, expression of hepatic inflammatory cytokines and intestinal tight junctional protein were analyzed, and the pathological changes of liver tissues were observed. The selected Lactobacillus strains could improve the liver injury of mice caused by chronic alcohol to a certain extent. Among them, Lactobacillus plantarum 45 (LP45) and Lactobacillus rhamnosus 519 (L519) significantly reduced liver fat accumulation and oxidative stress caused by chronic alcohol, inhibited the increase of serum enzymes and endotoxin, lowered the elevation of inflammatory factors in the liver, as well as improved the decrease in intestinal tight junction protein expression. In conclusion, LP45 and L519 could effectively alleviate chronic alcohol-induced liver injury in mice by improving oxidative stress and intestinal barrier function.

Key words： Lactobacillus; alcoholic liver disease; oxidative stress; intestinal barrier

Cite this article

ZHU Shiya , ZHAI Qixiao , ZHAO Xing , SUN Xinkai , LU Jianghao , LI Huawen , ZHAO Jianxin , ZHANG Hao , TIAN Fengwei , CHEN Wei . Comparison of the alleviation effects of different Lactobacilli on chronic alcohol-induced liver injury[J]. Food and Fermentation Industries, 2019 , 45(22) : 20 -26 . DOI: 10.13995/j.cnki.11-1802/ts.021066

References

[1] GAO B, BATALLER R. Alcoholic liver disease: Pathogenesis and new therapeutic targets[J]. Gastroenterology, 2011, 141(5): 1 572-1 585.
[2] 高潇雪,刘立新. 酒精性肝病流行病学及发病机制研究进展[J]. 中华消化病与影像杂志(电子版), 2016, 6(2): 62-65.
[3] 窦慧馨,张得钧. 酒精性肝病分子发病机制研究进展[J]. 基因组学与应用生物学, 2016,35(7): 1 643-1 647.
[4] 邱萍,李相,孔德松,等. 酒精性肝病发病机制研究的新进展[J]. 中国药理学通报, 2014, 30(2): 160-163.
[5] 刘国涛,朱玉翠,张涛,等. 酒精性肝病研究进展[J]. 世界华人消化杂志, 2017, 25(15): 1 382-1 388.
[6] SUGIMOTO K, TAKEI Y. Pathogenesis of alcoholic liver disease[J]. Hepatology Research, 2017, 47(1): 70-79.
[7] PUROHIT V, BODE J C, BODE C, et al. Alcohol, intestinal bacterial growth, intestinal permeability to endotoxin, and medical consequences: Summary of a symposium[J]. Alcohol, 2008, 42(5): 349-361.
[8] PARLESAK A, SCHAFER C, SCHUTZ T, et al. Increased intestinal permeability to macromolecules and endotoxemia in patients with chronic alcohol abuse in different stages of alcohol-induced liver disease[J]. Journal of Hepatology, 2000, 32(5): 742-747.
[9] 张娟,陈坚. 益生菌功能开发及其应用性能强化[J]. 科学通报, 2019,64(3): 246-259.
[10] KIRPICH I A, SOLOVIEVA N V, Leikhter S N, et al. Probiotics restore bowel flora and improve liver enzymes in human alcohol-induced liver injury: A pilot study[J]. Alcohol, 2008, 42(8): 675-682.
[11] HONG M, KIM S W, HAN S H, et al. Probiotics (Lactobacillus rhamnosus R0011 and acidophilus R0052) reduce the expression of toll-like receptor 4 in mice with alcoholic liver disease[J]. Plos One, 2015, 10(2):e0117 451.
[12] KIM W G, KIM H I, KWON E K, et al. Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 mitigate alcoholic steatosis in mice by inhibiting LPS-mediated NF-κB activation through restoration of the disturbed gut microbiota[J]. Food & Function, 2018(8): 9.
[13] TIAN F W, CHI F F,WANG G G, et al. Lactobacillus rhamnosus CCFM1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding[J]. Journal of Microbiology, 2015, 53(12):856-863.
[14] ZHE Z, HUI Z, LU B, et al. Protective effects of probiotics on acute alcohol-induced liver injury in mice through alcohol metabolizing enzymes activation and hepatic TNF-α response reduction [J]. Journal of Functional Foods, 2019, 59:234-241.
[15] 赵红霞,詹勇,许梓荣. 乳酸菌的研究及其应用[J]. 江西饲料, 2003(1).
[16] FORSYTH C B, FARHADI A, JAKATE S M, et al. Lactobacillus GG treatment ameliorates alcohol-induced intestinal oxidative stress, gut leakiness, and liver injury in a rat model of alcoholic steatohepatitis[J]. Alcohol, 2009, 43(2): 163-172.
[17] 黄亚娟,宋萌萌,孙琳. 不同来源乳酸杆菌生物学特性的比较[J]. 西北农林科技大学学报(自然科学版), 2011, 39(9):193-199.
[18] 蒙月月,赵莉,霍贵成. 体外评估十株乳杆菌对免疫细胞活性的影响[J]. 食品工业科技, 2018, 39(1):312-317.
[19] 赵林森,齐世华,马新颖, 等. 一种植物乳杆菌及其应用:中国, 201310587738.8[P]. 2014-03-19.
[20] 臧凯丽,江岩,孙勇,等.益生菌剂调整肠道疾病人群菌群结构丰度水平的研究[J]. 食品科学, 2018, 39(13): 133-143.
[21] 沈曦,何方,张玲琳. 植物乳杆菌LP45对OVA致敏小鼠的抗敏作用研究[C]//营养与食品科技学术年会暨“营养食品安全健康”成都论坛,2014.
[22] 蒲芳芳,万群,张瑜杰, 等. 植物乳杆菌LP45缓解抗生素对生命早期小鼠免疫及肠道菌群的影响[C]//益生菌:技术及产业化——第十三届益生菌与健康国际研讨会,2018.
[23] WANG Y, LIU Y, KIRPICH I, et al. Lactobacillus rhamnosus GG reduces hepatic TNF alpha production and inflammation in chronic alcohol-induced liver injury[J]. Journal of Nutritional Biochemistry, 2013, 24(9): 1 609-1 615.
[24] 吕圭源,陈素红,张丽丹, 等. 铁皮石斛对小鼠慢性酒精性肝损伤模型血清2种转氨酶及胆固醇的影响[J]. 中国实验方剂学杂志, 2010, 16(6):192-193.
[25] WU D, CEDERBAUM A I. Oxidative stress and alcoholic liver disease[J]. Seminars in Liver Disease, 2009, 29(2):141-154.
[26] DRAPER H H,HEDLEY M. Malondialdehyde determination as index of lipid peroxidation[J]. Methods Enzymol, 1990, 186:421-431.
[27] 龙建纲,王学敏,高宏翔,等. 丙二醛对大鼠肝线粒体呼吸功能及相关脱氢酶活性影响[J]. 第二军医大学学报, 2005, 26(10):1 131-1 135.
[28] 于洋,吴高峰,胡建民. 酒精性肝病的内毒素信号通路研究[J]. 黑龙江畜牧兽医, 2014(11):42-44.
[29] 冯彩珠,朱肖鸿. 酒精性肝病LPS信号通路与治疗的研究进展[J]. 浙江医学, 2016, 38(8):591-594.
[30] GUO F F,ZHENG K,RAQVEL B U, et al. The Lieber-De Carli diet-a flagship model for experimental alcoholic liver disease (ALD)[J]. Alcoholism: Clinical and Experimental Research, 2018,42(Suppl 2):1-7.
[31] MCCLAIN C, BARVE S, DEACIUC I, et al. Cytokines in alcoholic liver disease[J]. Seminars in Liver Disease, 1999, 19(2):205-219.
[32] 陈意,李方方,张勇,等. 肠黏膜上皮组织紧密连接的生物学功能和作用机理[J]. 动物营养学报, 2017, 29(9): 3 068-3 074.
[33] 王希,廖吕钊,江荣林. 肠上皮细胞紧密连接蛋白的结构功能及其调节[J]. 浙江医学, 2018, 40(8): 895-898.
[34] 夏婷,张瑾,姚佳惠,等.氧化应激在酒精性肝病中作用机制的研究进展[J]. 中国药理学通报, 2017,33(10):1 353-1 356.
[35] ZHONG W, MCCLAIN C J, CAVE M, et al. The role of zinc deficiency in alcohol-induced intestinal barrier dysfunction[J]. AJP: Gastrointestinal and Liver Physiology, 2010, 298(5): G625-G633.
[36] WANG Y, KIRPICH I, LIU Y, et al. Lactobacillus rhamnosus GG treatment potentiates intestinal hypoxia-inducible factor, promotes intestinal integrity and ameliorates alcohol-induced liver injury[J]. The American Journal of Pathology, 2011, 179(6): 2 866-2 875.
[37] MADSEN K, CORNISH A, SOPER P, et al. Probiotic bacteria enhance murine and human intestinal epithelial barrier function[J]. Gastroenterology, 2001, 121(3): 580-591.
[38] FABIA R, AR RAIAB A, JOHANSSON M L, et al. The Effect of exogenous administration of Lactobacillus reuteri R2LC and oat fiber on acetic acid-Induced colitis in the rat[J]. Scandinavian Journal of Gastroenterology, 1993, 28(2): 155-162.
[39] NANJI A A, KHETTRE U, SADRZAADEH S M H. Lactobacillus feeding reduces endotoxemia and severity of experimental alcoholic liver (disease)[J]. Proceedings of the Society for Experimental Biology & Medicine Society for Experimental Biology & Medicine, 1994, 205(3): 243-247.
[40] MIYAUCHI E, MORITA H, TANABE S. Lactobacillus rhamnosus alleviates intestinal barrier dysfunction in part by increasing expression of zonula occludens-1 and myosin light-chain kinase in vivo[J]. Journal of Dairy Science, 2009, 92(6): 2 400-2 408.
[41] WANG Y, LIU Y, SIDHU A, et al. Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury[J]. American Journal of Physiology Gastrointestinal and Liver Physiology, 2012, 303(1): 32-41.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.