The purpose of this study was to enhance exopolysaccharide (EPS) yield from Bifidobacterium lactis BB12® (BB12) and to determine the antioxidant activity of EPS. Single factor experiment was used to analyze the effects of initial pH, temperature and time on the yield of EPS. Subsequently, the fermentation parameters were further improved using response surface methodology. The antioxidant activity was determined by ferric reducing power of EPS, and the -OH and -O2 scavenging rate. The results showed that initial pH of 8, temperature of 37 ℃, and incubation time of 101 h was the optimum processing condition for producing EPS. Finally, the yield of EPS reached 131.6±0.82 mg/L. In addition, the reducing ability of EPS was further evaluated using in vitro antioxidant test, which IC50 -OH and -O2 was lower than that of Vc. Our research provided a reliable theoretical basis for the EPS commercial scale production using BB12, as well as foundations of understanding for antioxidant mechanism.
XIE Ying
,
CAI Guolin
,
LIU Yifan
,
LU Jian
. Optimization of fermentation conditions and antioxidant activities of exopolysaccharide from Bifidobacterium lactis Bb12®[J]. Food and Fermentation Industries, 2019
, 45(23)
: 55
-59
.
DOI: 10.13995/j.cnki.11-1802/ts.021708
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