An analytical method for rapid determination of enrofloxacin, ciprofloxacin, ofloxacin, pefloxacin, norfloxacin, lomefloxacin and sarafloxacin in poultry eggs by QuEChERS and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The quinolone residues in poultry eggs were extracted by 5% (V/V) formic acid-acetonitrile mixed solution, purified by QuEChERS and concentrated by a rapid solvent evaporation system, and separated on a C18 column with a mobile phase consisted of 0.1% (V/V) formic acid solution and acetonitrile by gradient elution. The quinolone residues were determined in a multiple reaction monitoring (MRM) mode by the electrospray ionization source (ESI+) and quantified with a matrix external standard method. The results showed that all the seven quinolones had good linearity in the concentration range of 2.0-100.0 ng/mL with the correlation coefficients greater than 0.995. The limits of detection (LOD) and limits of quantitation (LOQ) of the method were 0.3 μg/kg and 1.0 μg/kg, respectively, the average recovery rate was 77.20%-98.47%, and the relative standard deviation was 1.5%-6.2%. This method is easy, accurate and sensitive, which is suitable for rapid detection of quinolone residues in poultry eggs.
HUANG Kun
,
FAN Xiaolong
,
CAO Qi
,
HAN Zhi
,
ZHANG Li
,
WANG Huixia
,
GUO Jia
. Rapid determination of 7 quinolone residues in poultry eggs using QuEChERS and ultra performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2019
, 45(16)
: 261
-265
.
DOI: 10.13995/j.cnki.11-1802/ts.020743
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