The antifungal activity of fermentation broth of Lysinibacillus fusiformis He14 was analyzed, and eight pathogenic fungi were selected in this study. The stabilities of the antifungal components during extraction, precipitation and macroporous resin adsorption processes were investigated, respectively. It was found that the fermentation broth of strain He14 had inhibitory effects on all the eight pathogenic fungi and the bioactive substances were stable under extreme conditions of temperature, pH and UV. Moreover, LX-60 macroporous resin showed the best capability to adsorb and desorb bioactive components and three mixed components obtained through silica gel column chromatography showed inhibitory effects against Botrytis cinerea, Phytophthora infestans, Alternaria solani, Thanatephorus cucumeris and Trichophyton mentagrophytes. Component Ⅱ exhibited the highest inhibition effect on P. infestans. It was concluded that L. fusiformis He14 has excellent traits in antipathogenic fungus, which provides an experimental basis for the application of L. fusiformis in the fields of biological control and medicine.
ZHENG Wen
,
YANG Xingbian
,
KANG Jichuan
,
LI Hongqing
. Antifungal activity of fermentation broth of Lysinibacillus fusiformis He14[J]. Food and Fermentation Industries, 2019
, 45(18)
: 22
-26
.
DOI: 10.13995/j.cnki.11-1802/ts.020905
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