To establish and analyze an oyster-sensitized rat model, female brown Norwegian rats (BN rats) were sensitized by intragastric and intraperitoneal injection using the oyster allergen Cra g 1. The allergic reaction and changes of body weight, body temperature, organ index and blood index of each group of BN rats were observed. The changes of antibody and histamine were detected by ELISA, while the changes of tissues and organs were observed by hematoxylin-eosin (HE) and toluidine blue staining. The results showed that all the experimental BN rats had allergic reaction, while the control group had no obvious symptoms. The body weight of the experimental group was significantly lower than that of control group, the organ index was significantly higher than that of control group, and the number of cells in the blood changed. In addition, the levels of antibodies and histamines in the experimental group were higher than those in the control group during the sensitization period. The visceral lesions and serious degranulation of intestinal mast cells were observed in experimental rats. In this study, Cra g 1 protein sensitized serum was obtained, and BN rats were proved to be a good animal sensitization model, which provides a good basis for studying the allergen sensitization mechanism and immune regulation in vivo.
ZHANG Jiangtao
,
ZHANG Ruixue
,
FANG Lei
,
WEI Ying
,
LING Kong
,
ZHOU Ming
,
GU Ruizeng
,
LU Jun
,
LI Guoming
. Establishment and analysis of sensitization model of oyster Cra g 1 protein in rats[J]. Food and Fermentation Industries, 2019
, 45(18)
: 73
-79
.
DOI: 10.13995/j.cnki.11-1802/ts.020908
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