Using corn gluten meal and soy protein as raw materials, the antioxidant activity of the hydrolysate was studied after the corn protein: soy protein content ratio was 7∶3. Firstly, Aspergillus oryzae and Alcalase synergistic hydrolysis of the mixture of corn protein and soy protein to prepare corn soy protein hydrolysate(CSPHs); then the DPPH free radical scavenging rate and metal ion (Fe2+) chelation ability of CSPHs were determined to analyze its antioxidant activity in vitro. Establish a model of oxidative stress of hydrogen peroxide (H2O2) to investigate the cellular antioxidant activity of CSPH. The results showed that CSPHs had strong antioxidant activity in vitro, and the synergistically hydrolyzed product CSPH4 had good cellular antioxidant activity when 10% mass fraction of Aspergillus oryzae was added and 0.8% mass fraction of Alcalase was added, the hydrolysis degree and soluble protein content of the hydrolysate were 46.10% and (73.04±1.68) mg/mL, the scavenging capacities for DPPH radical and the chelating capacity of ferrous ions of the hydrolysate were (41.26±0.69)% and (50.23±3.15)%, respectively. CSPH4 could reduce the content of ROS in Caco-2 cells induced by H2O2-induced oxidative stress. The products obtained by the synergistic hydrolysis of Aspergillus oryzae and Alcalase have excellent antioxidant activity and intracellular ROS scavenging ability, which has the potential to be used as an antioxidant in the food industry.
MA Rui
,
LIU Xiang
,
LIN Wei
,
WANG Xiaojie
,
ZHENG Xiqun
,
LIU Xiaolan
. Preparation and antioxidant activity evaluation of hydrolysate from corn gluten meal and soy isolate protein by Aspergillus oryzae and alcalase[J]. Food and Fermentation Industries, 2019
, 45(19)
: 52
-57
.
DOI: 10.13995/j.cnki.11-1802/ts.020957
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