A rapid and sensitive method for determining N-acetylneuraminic acid (Neu5AC) in milk powder by ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) was established. The qualitative and quantitative analysis were achieved by multiple reactions, monitoring Neu5AC ion pair: 308.070 7→87.000 7, using electrospray ionization in negative ion mode. The results showed that in the range of 1.56-50.0 g/mL, the linear relationship of Neu5AC under optimal conditions was good (R2=0.999). The average recovery of milk powders was 86.79%-93.64% and RSD was 3.78%-6.42%. The detection limits, quantitation limits, and absolute value of matrix effect were 0.05 g/g, 0.3 g/g, and 14.14%, respectively. As a result, this method is simple in pretreatment, with high accuracy, sensitivity and repeatability, which can be effectively used for analysis and detection of Neu5AC in milk powder.
REN Yanjiao
,
LIU Yushu
,
WU Yao
,
YU Hui
,
LI Weili
,
WU Tao
. Evaluation of N-acetylneuraminic acid in milk powder by ultra-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry[J]. Food and Fermentation Industries, 2019
, 45(19)
: 287
-291
.
DOI: 10.13995/j.cnki.11-1802/ts.020823
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