This study revealed the correlation between the fermentation period and the quality of Chinese strong aroma-type Baijiu during five-month fermentation. The key flavor compounds and microbial community in fermented grains at the bottom layer were analyzed. It was found that lactic acid and acetic acid increased rapidly within 14 d of fermentation and followed by a moderate increase. Caproic acid, butyric acid and ethyl caproate gradually increased during fermentation. Lactobacillaceae sp. was dominant throughout the whole fermentation process, and the relative abundances of Ruminococcaceae sp. and Clostridiaceae_1 sp., two families with high abundances in pit muds, gradually increased. These results indicated that pit muds continuously provided anaerobic microorganisms for fermented grains during long-term fermentation, and these microorganisms played key roles in production of caproic acid, butyric acid and acetic acid. Although the abundances of anaerobic microorganisms originated from pit mud were low in fermented grains, they essentially contributed the flavor of strong aroma-type Baijiu, which provides a vital reference to control the quality of raw strong aroma-type Baijiu.
GAO Jiangjing
,
REN Cong
,
LIU Guoying
,
GE Xiangyang
,
WANG Huilin
,
YE Fangping
,
XU Yan
. Dynamic changes of flavor compounds and microbial community in fermented grains of Chinese strong aroma-type Baijiu during fermentation[J]. Food and Fermentation Industries, 2019
, 45(20)
: 1
-6
.
DOI: 10.13995/j.cnki.11-1802/ts.021271
[1] 张德才,王树龙,刘兴照.浓香型大曲酒生产双轮底工艺浅议[J].酿酒,1996(6):5-6.
[2] 李大和.浓香型白酒生产工艺与质量关系的思考[J].酿酒科技,2001(5):28-31.
[3] 栗连会,肖辰,陆震鸣,等.泸型酒发酵酒醅中乳酸菌群落的来源、演替规律及功能预测[J].食品与生物技术学报,2018,37(12):1 242-1 247.
[4] 肖辰,陆震鸣,张晓娟,等.泸型酒酒醅细菌群落的发酵演替规律[J].微生物学报,2019,59(1):195-204.
[5] 王鹏,吴群,徐岩.中国白酒发酵过程中的核心微生物群及其与环境因子的关系[J].微生物学报,2018,58(1):142-153.
[6] 刘凡,周新虎,陈翔,等.洋河浓香型白酒发酵过程酒醅微生物群落结构解析及其与有机酸合成的相关性[J].微生物学报,2018,58(12):2 087-2 099.
[7] TAO Y,LI J,RUI J,et al.Prokaryotic communities in pit mud from different-aged cellars used for the production of Chinese strong-flavored liquor[J].Appl Environ Microbiol,2014,80(7):2 254-2 260.
[8] HU X L,DU H,REN C,et al.Illuminating anaerobic microbial community and cooccurrence patterns across a quality gradient in Chinese liquor fermentation pit muds[J].Appl Environ Microbiol,2016,82(8):2 506-2 515.
[9] WANG C D,CHEN Q,WANG Q,et al.Long-term batch brewing accumulates adaptive microbes, which comprehensively produce more flavorful Chinese liquors[J].Food Research International,2014,62:894-901.
[10] 王明跃,张文学,王海英,等.不同窖龄窖泥古菌的系统发育多样性分析[J].应用与环境生物学报,2012,18(6):1 043-1 048.
[11] 刘森,李林光,李可,等.中国浓香型白酒窖池窖泥中原核微生物群落的空间异质性[J].食品科学,2013,34(21):221-226.
[12] 刘茂柯,唐玉明,赵珂,等.浓香型白酒窖泥微生物群落结构及其选育应用研究进展[J].微生物学通报,2017,44(5):1 222-1 229.
[13] WANG X,DU H,XU Y.Source tracking of prokaryotic communities in fermented grain of Chinese strong-flavor liquor[J].International journal of food microbiology,2017,244:27-35.
[14] 马雷,沈小梅,李安军,等.超高效液相色谱法测定白酒中乳酸的含量[J].食品与发酵科技,2016, 52(1):84-86.
[15] 任聪,辜杨,杜海,等.基于新老窖泥的微生物菌群结构判定浓香型白酒生产中的主体己酸菌[J].食品与发酵工业,2018,44(12):8-14.
[16] FAN W L,QIAN M C.Characterization of aroma compounds of Chinese "Wuliangye" and "Jiannanchun" liquors by aroma extract dilution analysis[J].Journal of Agricultural and Food Chemistry,2006,54(7):2 695-2 704.
[17] FAN W L,QIAN M C.Identification of aroma compounds in Chinese "Yanghe Daqu" liquor by normal phase chromatography fractionation followed by gas chromatography olfactometry[J].Flavour and Fragrance Journal,2006,21(2):333-342.
[18] WANG X,FAN W,XU Y.Comparison on aroma compounds in Chinese soy sauce and strong aroma type liquors by gas chromatography-olfactometry, chemical quantitative and odor activity values analysis[J].European Food Research and Technology,2014,239(5):813-825.
[19] 刘博,杜海,王雪山,等.基于高通量测序技术解析浓香型白酒中窖泥臭味物质4-甲基苯酚的来源[J].微生物学通报,2017,44(1):108-117.
[20] XU P X,CHAI L J,QIU T,et al.Clostridium fermenticellae sp. nov., isolated from the mud in a fermentation cellar for the production of the Chinese liquor, baijiu[J].International Journal of Systematic and Evolutionary Microbiology,2019,69(3):859-865.
[21] DU H,LIU B,WANG X,et al.Exploring the microbial origins of p-cresol and its co-occurrence pattern in the Chinese liquor-making process[J].International Journal of Food Microbiology,2017,260:27-35.