Structure and diversity of culturable endophytic bacteria in natural spice Cinnamomum longepaniculatum (Gamble) N. Chao leaves

  • ZHOU Wanhai ,
  • YANG Liyan ,
  • LI Miao ,
  • HUANG Jinfeng ,
  • LIANG Yujuan ,
  • WANG Xin ,
  • LUO Sican ,
  • WEI Qin ,
  • LIU Yang
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  • 1(Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education,Yibin University, Yibin 644000, China);
    2(School of Chemical and Bioengineering, University of Science and Technology Beijing, Beijing 100083, China)

Received date: 2019-07-03

  Online published: 2019-12-20

Abstract

The purpose of this study was to investigate the composition of endophytic bacterial communities in Cinnamomum longepaniculatum (Gamble) N. Chao leaves. A total of 166 representative strains were selected by morphological observation, followed by performing phylogenetic and diversity analyses based on 16S rDNA sequence. The results revealed that the population of endophytic bacteria varied between 0.7×103 and 4.5×104 CFU/g in leaves collected in different seasons, with the largest population observed in autumn. Strains identified were divided into 3 phyla, 13 genera, and 40 species. Nine genera and 23 species were identified in spring, four genera and four species identified in summer, five genera and 11 species found in autumn, and one genus and two species found in winter. Additionally, strains from genus Bacillus was the dominant bacteria in all samples. The leaves collected in autumn had abundant species, and the dominant genera were prominent and evenly distributed. In comparison, the diversity, dominance and evenness of endophytic bacteria in winter leaves were low. This study provides the groundwork for further exploration of the interactions between Cinnamomum longepaniculatum (Gamble) N. Chao leaves and symbiotic endophytic bacteria, as well as for investigating its metabolites and controlling essential oil synthesis.

Cite this article

ZHOU Wanhai , YANG Liyan , LI Miao , HUANG Jinfeng , LIANG Yujuan , WANG Xin , LUO Sican , WEI Qin , LIU Yang . Structure and diversity of culturable endophytic bacteria in natural spice Cinnamomum longepaniculatum (Gamble) N. Chao leaves[J]. Food and Fermentation Industries, 2019 , 45(20) : 43 -51 . DOI: 10.13995/j.cnki.11-1802/ts.021539

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