Food and Fermentation Industries >

2019 , Vol. 45 >Issue 20: 52 - 60

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.021548

Construction and performance evaluation of sodium alginate and konjac glucomannan microcapsules of multi-probiotics

  • ZHOU Yanlin ,
  • WU Ying ,
  • MA Yuhao ,
  • YAN Jiaqi ,
  • WANG Dahong ,
  • ZHANG Hongmei ,
  • GU Shaobin
Expand
  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China);
    2(Henan Engineering Research Center of Food Microbiology, Luoyang 471023, China);
    3(National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471023, China)

Received date: 2019-07-03

  Online published: 2019-12-20

Fold

Abstract

In order to improve the survival rates of Bifidobacteria, Lactobacillus plantarum and Lactobacillus casei after passing through the gastrointestinal tract, these strains were embedded in the microcapsules prepared by internal emulsification with konjac glucomannan (KGM) and sodium alginate. The optimal technological parameters for preparing the microcapsules were as follows: 3% ALG (w/w), 0.6% KGM (w/w), the volume ratio of water to oil was 1∶3, and the mass ratio of CaCO3 to ALG was 1∶3. Under this condition, the embedding rate reached (76.2±5.1)%. Moreover, the microcapsules not only improved the survival rate of probiotics in simulated gastric juice, but also enhanced their tolerances to bile salts. The results of serum biochemical parameters and histopathological observation showed that the material was not toxic to mice. After intragastric administration of KGM, the relative abundances of Lactobacillaceae sp. increased and Desulfovibrionaceace sp. decreased, which indicated that KGM could promote the growth of beneficial bacteria and inhibit harmful bacteria. This research expanded a new field for the application of KGM, and the results provide a reference for industrial production and application of compound probiotic microencapsulation.

Key words： probiotic; konjac glucomannan; microencapsulation; internal emulsification method; intestinal probiotics

Cite this article

ZHOU Yanlin , WU Ying , MA Yuhao , YAN Jiaqi , WANG Dahong , ZHANG Hongmei , GU Shaobin . Construction and performance evaluation of sodium alginate and konjac glucomannan microcapsules of multi-probiotics[J]. Food and Fermentation Industries, 2019 , 45(20) : 52 -60 . DOI: 10.13995/j.cnki.11-1802/ts.021548

References

[1] ROKKA S, RANTAMAKI P. Protecting probiotic bacteria by microencapsulation: Challenges for industrial applications[J]. European Food Research & Technology, 2010,231(1):1-12.
[2] DE CASTROCISLAGHI F P, CARINADOS REIS E S,FRITZEN-FREIRE C B, et al. Bifidobacterium Bb-12 microencapsulated by spray drying with whey: Survival under simulated gastrointestinal conditions, tolerance to NaCl, and viability during storage[J]. Journal of Food Engineering, 2012,113(2):186-193.
[3] 赵萌,蔡沙,屈方宁,等. 内源乳化法制备海藻酸盐微胶囊的研究进展[J]. 食品工业科技, 2013,34(22):392-396.
[4] KHANNA S, TESTER R F. Influence of purified konjac glucomannan on the gelatinisation and retrogradation properties of maize and potato starches[J]. Food Hydrocolloids, 2006, 20(5):567-576.
[5] CHUA M, BALDWIN T C, HOCKING T J, et al. Traditional uses and potential health benefits of Amorphophallus konjac K. Koch ex N.E.Br.[J]. Journal of Ethnopharmacology, 2010, 128(2):268-278.
[6] CONNOLLY M L, LOVEGROVE J A, TUOHY K M, et al. Konjac glucomannan hydrolysate beneficially modulates bacterial composition and activity within the faecal microbiota[J]. Journal of Functional Foods, 2010, 2(3):219-224.
[7] CHEN H L, CHENG H C, WU W T, et al. Supplementation of konjac glucomannan into a low-fiber chinese diet promoted bowel movement and improved colonic ecology in constipated adults: A placebo-controlled, diet-controlled trial[J]. Journal of the American College of Nutrition, 2008, 27(1):102-108.
[8] NISHINARI K, WILLIAMS P A, PHILLIPS G O, et al. Review of the physico-chemical characteristics and properties of konjac mannan[J]. Food Hydrocolloids, 1992,6(2):199-222.
[9] WEN X, WANG T, WANG Z, et al. Preparation of konjac glucomannan hydrogels as DNA-controlled release matrix.[J]. International Journal of Biological Macromolecules, 2008,42(3):256-263.
[10] WANG K, HE Z. Alginate–konjac glucomannan–chitosan beads as controlled release matrix[J]. International Journal of Pharmaceutics, 2002,244(1-2):117-126.
[11] WAN B R, XIA B, LI B J, et al. Semi permeable nanocapsules of konjac glucomannan–chitosan for enzyme immobilization[J]. International Journal of Pharmaceutics, 2008,364(1):102-107.
[12] CHANDRAMOULI V, KAILASAPATHY K, PEIRIS P, et al. An improved method of microencapsulation and its evaluation to protect Lactobacillus spp. in simulated gastric conditions[J]. Journal of Microbiological Methods, 2004,56(1):27-35.
[13] ZOU Q, ZHAO J, LIU X, et al. Microencapsulation of Bifidobacterium bifidum F-35 in reinforced alginate microspheres prepared by emulsification/internal gelation[J]. International Journal of Food Science & Technology, 2011,46(8):1 672-1 678.
[14] GANI A, SHAH A, AHMAD M, et al. β-D-glucan as an enteric delivery vehicle for probiotics[J]. International Journal of Biological Macromolecules, 2018,106:864-869.
[15] 赵萌,蔡沙,屈方宁,等. 海藻酸钠-魔芋葡甘聚糖微胶囊对嗜酸乳杆菌CGMCC1.2686保护研究[J]. 现代食品科技, 2015,31(2):70-75.
[16] MENG Z, QU F, SHA C, et al. Microencapsulation of Lactobacillus acidophilus CGMCC1.2686: Correlation between bacteria survivability and physical properties of microcapsules[J]. Food Biophysics, 2015,10(3):292-299.
[17] KUO C K, MA P X. Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: Part 1. Structure, gelation rate and mechanical properties[J]. Biomaterials, 2001,22(6):511-521.
[18] LEROUX M A, GUILAK F, SETTON L A, et al. Compressive and shear properties of alginate gel: Effects of sodium ions and alginate concentration[J]. Journal of Biomedical Materials Research, 2015,47(1):46-53.
[19] 宋娇娇,包秋华,王亚利,等. 瑞士乳杆菌MG9-2微胶囊的制备工艺优化及其性能分析[J]. 食品科学, 2017,38(14):56-64.
[20] SILVA C M, RIBEIRO A J, FIGUEIREDO I V, et al. Alginate microspheres prepared by internal gelation: Development and effect on insulin stability[J]. International Journal of Pharmaceutics, 2006,311(1-2):1-10.
[21] SULTANA Y, MALL S, MAURYA D P, et al. Preparation and in vitro characterization of diltiazem hydrochloride loaded alginate microspheres[J]. Pharmaceutical Development and Technology, 2009, 14(3):321-331.
[22] LEE B J, MIN G H, KIM T W, et al. Preparation and in vitro release of melatonin-loaded multivalent cationic alginate beads[J]. Archives of Pharmacal Research, 1996,19(4):280-285.
[23] TAKKA S, OCAKOH, ACARTURK, et al. Formulation and investigation of nicardipine HCl-alginate gel beads with factorial design-based studies[J]. European Journal of Pharmaceutical Sciences, 1998, 6(3):241-246.
[24] MIRGHANI A, IDKAIDEK N M, SALEM M S, et al. Formulation and release behavior of diclofenac sodium in compritol 888 matrix beads encapsulated in alginate[J]. Drug Development & Industrial Pharmacy, 2000, 26(7):791-795.
[25] CHEN M, MUSTAPHA A. Survival of freeze-dried microcapsules of α-galactosidase producing probiotics in a soy bar matrix[J]. Food Microbiology, 2012, 30(1):68-73.
[26] HOMAYOUNI A, AZIZI A, EHSANI M R, et al. Effect of microencapsulation and resistant starch on the probiotic survival and sensory properties of synbiotic ice cream[J]. Food Chemistry, 2008, 111(1):50-55.
[27] KRASAEKOOPT W, BHANDARI B, DEETH H, et al. The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria[J]. International Dairy Journal, 2004, 14(8):737-743.
[28] DE CASTROCISLAGHI F P, CARINA DOAREIS E S, FRITZEN-FREIRE C B, et al. Bifidobacterium Bb-12 microencapsulated by spray drying with whey: Survival under simulated gastrointestinal conditions, tolerance to NaCl, and viability during storage[J]. Journal of Food Engineering, 2012, 113(2):186-193.
[29] ANDRIAMANANTOANINA H, RINAUDO M. Relationship between the molecular structure of alginates and their gelation in acidic conditions[J]. Polymer International, 2010,59(11):1 531-1 541.
[30] 黄夏伶,张炜,李玉桑. 魔芋葡甘聚糖对小鼠肠道生理结构及蠕动功能的影响[J]. 现代食品科技, 2018, 34(4):7-11.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.