In order to investigate the anti-aging and anti-oxidant ability of lively and sterilized fermented Hovenia dulcis juices, the effects of fermented Hovenia dulcis juices on the in vivo and in vitro antioxidant capacity and the life span of Drosophila melanogaster were comprehensively analyzed. The results showed that the median lethal time (LT50), mean life-span (MLS) and survival rate of male and female Drosophila melanogaster increased with increasing feeding dose of lively and sterilized fermented juices. When fed with 15 mg/mL lively fermented juice, the MLS of male and female Drosophila melanogaster was prolonged by 66.49% and 61.16% respectively, compared with CK group, and sterilized fermented juice fed group was prolonged by 48.87% and 47.69%, respectively. The activities of T-SOD in male and female Drosophila melanogaster fed with lively fermented juice for 20 d were 429.85 and 431.53 U/mg prot, respectively, while that of male and female Drosophila melanogaster fed with sterilized fermented juice were 390.81 and 397.77 U/mg prot, respectively. In conclusion, Lactobacillus fermented juice was anti-oxidative, and could prolong the life span and improve the survival rate of Drosophila melanogaster, which provides a theoretical basis for developing products of fermented Hovenia dulcis juice.
WANG Zihan
,
QIAN Zilin
,
XIANG Min
,
XU Mao
,
JIANG Heti
. Effects of Lactobacillus fermented Hovenia dulcis juice on life-span and antioxidant activity of Drosophila melanogaster[J]. Food and Fermentation Industries, 2019
, 45(20)
: 112
-117
.
DOI: 10.13995/j.cnki.11-1802/ts.021447
[1] 赵国平.中药大辞典[M].上海:上海科学技术出版社, 2009.
[2] HYUN T K, EOM S H, YU C Y, et al. Hovenia dulcis-an Asian traditional herb[J]. Planta Medica, 2010,76(10):943-949.
[3] 王婷婷,李华,王华,等.拐枣的功效成分及加工利用[J].中国食物与营养,2018,24(10):21-26.
[4] 李寒冰,吴宿慧,王凌潇,等.鲜拐枣汁对酒精致急性肝损伤小鼠的保护作用研究[J].中药药理与临床,2018,34(4):110-115.
[5] 何涛,杜瀛琨,蓝伦礼,等.枳椇子的研究概况[J].云南中医中药杂志, 2009,30(5):64-66.
[6] LEBEER S, VANDERLEYDEN J,DE KEERSMAECKER S C J. Genes and molecules of lactobacilli supporting probiotic action[J]. Microbiol Mol Biol Rev, 2008,72(4):728-764.
[7] 丘裕.益生菌果蔬汁的研究进展[J].现代食品, 2016,7(13):70-73.
[8] 王超,林小晖,杜冰.乳酸菌发酵型饮料的研究现状与前景[J].饮料工业, 2018,21(1):68-70.
[9] 陈历水,丁庆波,吴伟莉,等.发酵果蔬汁的功能特性研究进展[J].食品工业科技, 2012,33(11):418-421.
[10] RUMBAOA R G O, CORNAGO D F, GERONIMO I M. Phenolic content and antioxidant capacity of Philippine sweet potato (Ipomoea batatas) varieties[J]. Food Chemistry, 2009,113(4):1 133-1 138.
[11] 范金波,蔡茜彤,冯叙桥,等.5种天然多酚类化合物抗氧化活性的比较[J].食品与发酵工业, 2014,40(7):77-83.
[12] 綦菁华,王有年,于同泉,等.不同品种桃的酚类活性成分及其抗氧化功能研究[J].食品与发酵工业, 2006,32(1):103-106.
[13] RE R, PELLEGRINI N, PROTEGGENTE A, et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay[J]. Free Radical Biology and Medicine, 1999,26(9-10):1 231-1 237.
[14] 潘丽军,孙潇雅,姜绍通.紫薯复合饮料的质量指标、营养成分及抗氧化性分析[J].食品科学, 2013,34(20):165-169.
[15] 张碧莹,钱籽霖,唐玲,等.甘薯汁对果蝇寿命及抗氧化能力的影响[J].食品科学,2019,40(5):197-201.
[16] 王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工, 2009,23(3):40-42.
[17] 中华人民共和国国家质量监督检验检疫总局. SN/T 3926-2014 出口乳、蛋、豆类食品中蛋白质含量的测定考马斯亮蓝法[S].北京:中国标准出版社, 2014.
[18] 陈纯.紫薯提取物对果蝇和线虫抗氧化作用的研究[D].天津:天津科技大学, 2016.
[19] 王娟,任莉萍,屈长青,等.香椿子对果蝇的寿命及其抗氧化相关指标的影响作用[J].天然产物研究与开发, 2015,27(11):1 858-1 861;1 865.
[20] 胡莎.根皮苷对果蝇抗衰老的机理研究[D].天津:天津科技大学, 2012.
[21] 张欣文,徐思红,厉曙光.果蝇体内SOD和MDA随增龄变化及其与寿命的关系[J].中国公共卫生, 2000,16(3):222-223.
