In order to find the microbial resources with antiseptic and fresh-keeping function, a strain of bacteria with antibacterial activity was isolated and purified from the pervious orange fresh-keeping study. The strain was identified as Bacillus amyloliquefaciens KHQH-1 by morphology, biochemistry and molecular biology. Through BLAST alignment on NCBI platform, sequence analysis and phylogenetic tree construction with Clustalw and Mega 7.0 software, the phylogenetic status of the strain was established. Point planting method and filter paper method were used to study the antagonistic effects of KHQH-1 on several common fungi and bacteria in food. The results showed that KHQH-1 had a certain inhibitory effect on Rhizopus spp. and Aspergillus oryzae. It had a strong inhibitory effect on the most common fungi in food, such as Aspergillus flavus, Penicillium spp. and Trichoderma viride. The inhibitory circles were 13.70, 9.30 and 10.87 mm, respectively. KHQH-1 has certain inhibitory effect on common bacteria Staphylococcus aureus and Escherichia coli in food, and its inhibitory circle was 3.20 and 8.20 mm, respectively. These results will provide some theoretical and technical reference, which can explore the preservation technology of natural products and the development and application of microbial preparations.
[1] 金红,叶锐,徐晶,等.抗菌肽对食品腐败菌抑制效果的研究进展[J].农产品加工,2018(3):59-63.
[2] 黄应维,徐匆,马锞,等.果蔬微生物保鲜技术的研究进展[J].现代食品科技,2013,29(6): 1 455-1 458.
[3] 蔡国林,冯文旭,刘逸凡,等.高产抑制大肠杆菌血凝性的胞外多糖的解淀粉芽孢杆菌[J].食品与发酵工业,2019,45(5):14-18.
[4] 尹珺伊,陈楠楠,王岩,等. 解淀粉芽胞杆菌SSY2分离鉴定及其拮抗霉菌特性研究[J].动物医学进展,2019,40(4):75-79.
[5] 陈建军,郝之奎,廖祥儒,等. Bacillus amyloliquefaciens MBRC1 的鉴定与抗菌活性研究[J]. 食品与生物技术学报,2019,38(2):126-132.
[6] SHAHZADI S,KHAN Z,REHMAN A,et al.Isolation and characterization of bacillus amyloliquefaciens 6a: A novel kerosene oil degrading bacterium[J].Environmental Technology & Innovation, 2019, 14: 100 359.
[7] 洪鹏,安国栋,胡美英, 等. 解淀粉芽孢杆菌防治果蔬采后病害研究进展[J]. 中国农学通报,2013, 29(12):168-173.
[8] CALVO H,MENDIARA I,ARIAS E, et al. The role of iturin A from B. amyloliquefaciens BUZ-14 in the inhibition of the most common postharvest fruit rots[J]. Food Microbiology, 2019, 82: 62-69.
[9] 耿阳阳,徐俐,刘昕,等. 解淀粉芽孢杆菌发酵液在鲜核桃保鲜中的应用[J]. 食品与发酵工业, 2013, 39(9):214-218.
[10] CUI W Y, HE P J, MUNIR S, et al. Efficacy of plant growth promoting bacteria Bacillus amyloliquefaciens B9601-Y2 for biocontrol of southern corn leaf blight[J]. Biological Control,2019,139:104 080.
[11] AGUSTIN L B, RAUNEL TV, GERARDO C, et al. Effects of bacillomycin D homologues produced by Bacillus amyloliquefaciens 83 on growth and viability of Colletotrichum gloeosporioides at different physiological stages[J]. Biological Control, 2018,127(12): 145-154.
[12] ZHANG Qingxia, ZHANG Ying, HE Lingling, et al. Identification of a small antimycotic peptide produced by Bacillus amyloliquefaciens 6256[J]. Pesticide Biochemistry and Physiology, 2018,150(9):78-82.
[13] 邝格灵,张洁,孔德华,等. 植物乳杆菌与解淀粉芽孢杆菌对食醋风味的影响[J].中国酿造,2018,37(6):25-29.
[14] 赵龙,周池虹伶,赵谋明,等.解淀粉芽孢杆菌SWJS22和米曲霉混合制曲在酱油发酵中的应用[J].食品科学, 2017,38(22):125-130.
[15] 孙燕萍,彭浩,凌霞,等. VITEK2Compact全自动微生物分析系统的应用及鉴定结果分析[J]. 现代预防医学,2010, 37(20):3 891-3 893.
[16] 林杰,张景艳,王磊,等.VITEK2 Compact全自动微生物分析系统对牛乳中葡萄球菌的鉴定效果评价[J].微生物学通报,2016, 43(11):2 514-2 520.
[17] 杨玉红,陈淑范.食品微生物学.[M].第2版.武汉:武汉理工大学出版社,2014.
[18] FRANK J A,REICH C I,SHARMA S,et al.Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes[J].Applied and Environmental Microbiology,2008,74(8):2 461-2 470.
[19] 付志英,刘彩珍,周红艳,等.纳豆菌拮抗黄曲霉试验方法的研究[J].中国调味品,2019,44(4):93-96.
[20] 权春善,王军华,徐洪涛,等.一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究[J].微生物学报,2006,46(1):7-12.
[21] 张超,王祥红,徐涛,等.一株解淀粉芽孢杆菌的分离鉴定及其抑菌条件研究[J].生物技术通报,2015,31(9):170-176.
[22] FELSENSTEIN J. Confidence limits on phylogenies: An approach using the bootstrap[J]. Evolution, 1985, 39(4): 783-791.
[23] 冯志珍.解淀粉芽胞杆菌FB-16的筛选及其对作物疫霉菌的抑制作用[D].杨凌:西北农林科技大学,2012.
[24] 刘超,刘洪伟,汪步青,等. 解淀粉芽孢杆菌BA-26抗菌物质分离及对灰葡萄孢抑菌作用研究[J]. 生物技术通报, 2019,35(7):83-89.
[25] 柴庆凯,张斌,常若葵,等.解淀粉芽孢杆菌LJ02对黄瓜抗灰霉病菌的生防效果及其诱导抗性机理的初步研究[J].植物病理学报,2019,49(6):828-835.
[26] 史一然,徐伟慧,吕智航,等. 解淀粉芽孢杆菌 LZN01 对西瓜专化型尖孢镰刀菌的抑制效应[J].江苏农业科学,2019,47(12): 141-145.
[27] 陈照,李培征,刘惠芳,等.解淀粉芽孢杆菌HAB-9的鉴定及对17株植物病原菌的拮抗作用[J]. 热带农业科学, 2019,39(7):47-52.
[28] 杨帆,张飞燕,孙劲冲,等.马铃薯真菌病害拮抗菌株鉴定及初步应用[J].中国蔬菜,2019(11):56-62.
