A headspace (HS)/solid phase microextraction (SPME)/gas chromatography-mass spectrometry (GC-MS) method was developed to accurately determine the profiles of volatile compounds from royal jelly based on the single-factor tests of extraction temperature and time, sample size, saturated NaCl solution addition. The optimization of the extraction conditions was carried out by response surface methodology. According to optimized extraction conditions, volatile compounds in royal jelly from five different sources were determined by gas chromatography-mass spectrometry (GC-MS). The analytical conditions of the optimized HS-SPME method were: extraction temperature 62 ℃, extraction time 60 min, 2 g royal jelly, 1 mL saturated NaCl solution. Under this extraction condition, the total peak area was the largest. The GC-MS analysis allowed the identification of 48 volatile compounds in five royal jelly. The 16 common volatile compounds in the samples were rich in acid, ester and aldehyde compounds, but their content was varied. These common compounds may be related to the characteristic flavor of royal jelly. The results could be used for the detection and application of volatile compounds in royal jelly.
WANG Jiao
,
LI Yuxin
,
ZHAO Baotang
,
HE Xingfen
,
ZHANG Jun
,
ZHANG Zhaoyun
,
YANG Fumin
. Optimization of HS-SPME/GC-MS method for determination of volatile components in royal jelly[J]. Food and Fermentation Industries, 2020
, 46(8)
: 239
-245
.
DOI: 10.13995/j.cnki.11-1802/ts.022968
[1] LIU J R, YUAN Y C, SHI L S, et al. Antioxidant properties of royal jelly associated with larval age and time of harvest[J].Agric Food Chem. 2008,56 (23): 11 447-11 452.
[2] SCARSELLI R,DONADIO E,GIUFFRIDA M G. Towards royal jelly proteome[J]. Proteomics, 2005,5: 769-776.
[3] ANKLAM E, RADOVIC B.Suitable analytical methods for determining the origin of European honey[J]. American Laboratory, 2001,33: 60-62.
[4] WALDMAR,WARDENLKL,MAGDLENA,et al. A review of theoretical and practical aspects of solid-phase microextraction in food analysis[J]. Institute of Food Science Techology, 2004,39:703-717.
[5] ISIDOROV V A, BAKIER S, GRZECH I. Gas chromatographic-mass spectrometric investigation of volatile and extractable compounds of crude royal jelly[J]. Journal of Chromatography B, 2012, 885:109-116.
[6] ZHAO Y Z, LI Z G, TIAN W L, et al.Differential volatile organic compounds in royal jelly associated with different nectar plants[J].Journal of Integrative Agriculture,2016,15(5):1 157-1 165.
[7] BOSELLI E, CABONI M F, SABATINI A G, et al. Determination and changes of free amino acids in royal jelly during storage[J]. Apidologie, 2003,34(2): 129-138.
[8] 任佳淼,彭文君,田文礼,等.加工工艺对蜂蜜挥发性成分的影响[J].食品科学,2014,35(10):41-45.
[9] CEBALLOS LIENA,PINO JORGE A, DAGO ANGEL, et al. Optimization of a HS-SPME/GC-MS method for determination of volatile compounds in some cuban unifloral honeys[J]. Food Quality,2010, 33(4):507-528.
[10] MIRHOSSEINI H, SALMAH Y, NAZIMAH S A H, et al.Solid-phase microextraction for headspace analysis of key volatile compounds in orange beverage emulsion[J]. Food Chemistry,2007,105: 1 659-1 670.
[11] ZHANG Y, GAO B, ZHANG M, et al. Headspace solid-phase microextraction-gas chromatography-mass spectrometry analysis of the volatile components of longan (Dimocarpus longan Lour.)[J]. Eurpean Food Research and Technology,2009,229:457-465.
[12] KING A J, READMAN J W, ZHOU J L, The application of solid-phase micro-extraction (SPME) to the analysis of polycyclic aromatic hydrocarbons (PAHs)[J]. Environ Geochem Health,2003, 25:69-75.
[13] MOREIRA N, MEIRELES S, BRANDÃO T,et al. Optimization of the HS-SPME-GC-IT/MS method using a central composite design for volatile carbonyl compounds determination in beers[J]. Talanta,2013, 117: 523-531.
[14] BALASUBRAMANIAN S, PANIGRAHI S, Solid-phase microextraction (SPME) techniques for quality characterization of food products: a review[J]. Food Bioprocess Technology,2011,4:1-26.
[15] BARONI M V, NORES M L, DIAZ M D P, et al. Determination of volatile organic compound patterns characteristics of five unifloral honeys by solid-phase microextraction-gas chromatography-mass spectrometry coupled to chemimetrics[J]. Journal of Agricultural and Food Chemistry, 2006,54(19): 7 235-7 241.
[16] MANYI-LOH C E, NDIP R N, CLARKE A M. Volatile compounds in honey: A review on their involvement in aroma, botanical origin determination and potential biomedical activities[J]. International Journal of Molecular Sciences,2011,12(12), 9 514-9 532.